General information for g9466.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g9466 is located on chr_1: 3424628..3427626 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9466 | g9466.t1 | TSS | g9466.t1 | 3424589 | 3424589 |
| chr_1 | g9466 | g9466.t1 | isoform | g9466.t1 | 3424628 | 3427626 |
| chr_1 | g9466 | g9466.t1 | exon | g9466.t1.exon1 | 3424628 | 3425773 |
| chr_1 | g9466 | g9466.t1 | cds | g9466.t1.CDS1 | 3424628 | 3425773 |
| chr_1 | g9466 | g9466.t1 | exon | g9466.t1.exon2 | 3425830 | 3426154 |
| chr_1 | g9466 | g9466.t1 | cds | g9466.t1.CDS2 | 3425830 | 3426154 |
| chr_1 | g9466 | g9466.t1 | exon | g9466.t1.exon3 | 3426300 | 3426377 |
| chr_1 | g9466 | g9466.t1 | cds | g9466.t1.CDS3 | 3426300 | 3426377 |
| chr_1 | g9466 | g9466.t1 | exon | g9466.t1.exon4 | 3426446 | 3427626 |
| chr_1 | g9466 | g9466.t1 | cds | g9466.t1.CDS4 | 3426446 | 3427626 |
| chr_1 | g9466 | g9466.t1 | TTS | g9466.t1 | 3427741 | 3427741 |
Sequences
>g9466.t1 Gene=g9466 Length=2730
ATGAAAAGTTTCATTATTGTGATTGCACTTATAAGTGTAATTTTAGCTAGTCCAATTGAG
AACACTCAAAAGTCATTCAAATATCAACAGTCAGATGAAATTCCAGTTGAATTTAAAGAG
TTTCTATTTAAAAATGGTGAAATTGTGCAAGAAGAAATGAAAATTCCAAAATTTAATGTC
GATAATGATGAAGATTATGTAACATATCGATTACCAAATAATACAAAACCAGTGAGATAT
GATTTATGGGTGATGACTGATGTTGAAAAAGAAGATTTCAATTTTACAGGTCGAGTAAAA
ATTCACATTCGTGCTATTGAAGAAACTGATTATGTGACATTAAATTATCGCGAGACTCAA
ATTGATTTGATTGATTTATTGAATGTCGAAACGAATCAAGTGATTGAAACAAATTTGACT
TATAACTTTCTTGAGCCTCTCGATCGTGAATTTATGAGAATTTCACTTCCATCAACTGTG
ACTAAAGACTCAGAATTTATTCTTGATATAAAATATCATGGTGTGCTGCATGTGGCTGAT
GATAATGGTTTTTATAGAGCCAGTTACAGAGATCAAAATACAAATGAGTTAGTCTGGTAT
GCTACAACCAAATTTGAGCCACATCATGCTCGTCATCTCATGCCATGCTATGATGAACCA
CAAATTCGTGCACCAATTGGATTACAAGTACAACATGATAAGAATTATCGAACATTTTCA
AATATGCCAATCATAAATCGTACTGAAATTGAAGGTACTGATTATGTGTTATCAATTTTT
ATTGACACTCCACCAATGCAGACTTATTTACTTGCATTTCTTGTCTCTGATTTTCAATAC
AAATCAAATGAAGCTCAAGACATTGAACAGAGAATTTTCGCCAAACCTCAATCGATTCAA
GACGGTTCAGCTGATTATGCAGCTTCGATATCTGATGAGATTTTAAAGAAATTTGAAGAA
ATCTTAAATGTTCCTTATATGCTACCAAAACTTGATCATGCAGCAATCACAGAATTTCCA
AGTGGTGCAATGGAAAATTTTGGATTTATAACTTATCGTGAAGTAGGCTTGCTGCTTTAT
ACAAATTATACTGAAGCACAAATTTACTCTAATAAAAAATACATCGCTAGATTATTAGCC
CATGAGATAACACATCAATGGTTTGGAAATTTAGTTTCTCCTCAATGGTGGACTTATCTC
TTTCTTAATGAAGGATTTGCAACATTCTTTCAATATTATGTTACTAATCATCTTTTCCCT
GAACTTGGTTTTGATGAAGACTATAGAACTGCTGCTATTCAAGCTTCTTTAGTCAACGAT
GTAATCACAAATCCAGCTTGTGTTCCAATGGAATATTATGTTGAAGAACAAACTGCCATT
CGTGGTCGATTCAATTATGTTTCATATCAAAAAGCAGGCAGCATAATTGGAATGTTTTTT
GAAGCAATTGGAGAGAAAATTTTCCTAAAAGCAGGTTATCCAATTGTATCGATTGAAAAG
TCTGGAAGCAAACTCAAATTTGAGTCAGACAATATTCCAAGTGGAAATGGACATTGGTTG
ACAACAAAGGAAGAAGTTAATTTCGAAAATCTCAATTTCAATGAAGGCGATTGGATAATT
TTGAATAACCAACAAGTTGGCTTTTATCGTGTCAGTTACAGTGATGAATTGTGGAAGGCA
ATTGCTAAAGGCTTGCAATCAAATCACAGACAAATTCATTTAATTAACCGTAGAATTTTA
CAAGAAGAACTCAACATTGGCATAAATGTACTTGATAAAGTTTCAGCATCAACAGTATTG
ACAATGTTAAAAGCAATAAAAAGTGAAGACAATTATCTTGTATGGAATGATGCAAATGTC
AATTTTCAATTACTTAATACAACACTTTTTGGAACAACCGCATATGATGATTACATGAAT
TTCATTCAAACATTGACTAAAGAAATTATTAATGAAATTGGATATGAAAGTATGGAATCA
GAATCATCAGAAATTACAAGTTTAAGAGCAAGAGTAAAATTGCTTAACTGTTATGCATTT
GATGAAACATGCATGCAACATGAACTCAATAAACTCATCAAATATCATGAAAATGAAGGA
GAAAATTTTGCACCAGATTTTTGCACTGCTTTTCGTCATGCAAATCAAACAATTTTCGAT
CATTATTTGAATCAACTTGTGTCAAATGTGACTTTAAAAAATCGAAATTTCATTGCAAGA
ACAATTCATTGTTCACTTAATGAAAATTATCTTGGTGTAATTGTAACTGCAATTGAAGAC
ACAACAAATATTTTAACTGATGCAGAAAGAATAAATTTAATTAACAACATGCTCATTTCA
AGTTCATCTGCTTTCAATGTTGCATTTGAATACATCAAGAGAAATGTCGATAAAATCGAT
TCATTTAGAGCAAGACTTATGACTGCGATCAACACACAAGAGAAATTCAATGAACTCAAA
TCGTTGCTCAATGAAGCAATCGATGAAGGAATTTTGACTCAAATTCAAGCAAATGAAACC
ATTGCTGCTATTGAGAAGAATTTAAAATGGCAAGAAAAGCATTTAGATGACATTAAAAAA
TGGTTTGNNNNNNNNNNNNNNNNNNAAGAAGAAGAAACAACAACGACTGCAACAGTTGCA
ACAACAATAGAAACAACAACACAAGGATCAAATGGAAAAATCATTTCATTTTATCTTGTC
TGTTTTTCAATCATTTTAAGCTACTTGTGA
>g9466.t1 Gene=g9466 Length=909
MKSFIIVIALISVILASPIENTQKSFKYQQSDEIPVEFKEFLFKNGEIVQEEMKIPKFNV
DNDEDYVTYRLPNNTKPVRYDLWVMTDVEKEDFNFTGRVKIHIRAIEETDYVTLNYRETQ
IDLIDLLNVETNQVIETNLTYNFLEPLDREFMRISLPSTVTKDSEFILDIKYHGVLHVAD
DNGFYRASYRDQNTNELVWYATTKFEPHHARHLMPCYDEPQIRAPIGLQVQHDKNYRTFS
NMPIINRTEIEGTDYVLSIFIDTPPMQTYLLAFLVSDFQYKSNEAQDIEQRIFAKPQSIQ
DGSADYAASISDEILKKFEEILNVPYMLPKLDHAAITEFPSGAMENFGFITYREVGLLLY
TNYTEAQIYSNKKYIARLLAHEITHQWFGNLVSPQWWTYLFLNEGFATFFQYYVTNHLFP
ELGFDEDYRTAAIQASLVNDVITNPACVPMEYYVEEQTAIRGRFNYVSYQKAGSIIGMFF
EAIGEKIFLKAGYPIVSIEKSGSKLKFESDNIPSGNGHWLTTKEEVNFENLNFNEGDWII
LNNQQVGFYRVSYSDELWKAIAKGLQSNHRQIHLINRRILQEELNIGINVLDKVSASTVL
TMLKAIKSEDNYLVWNDANVNFQLLNTTLFGTTAYDDYMNFIQTLTKEIINEIGYESMES
ESSEITSLRARVKLLNCYAFDETCMQHELNKLIKYHENEGENFAPDFCTAFRHANQTIFD
HYLNQLVSNVTLKNRNFIARTIHCSLNENYLGVIVTAIEDTTNILTDAERINLINNMLIS
SSSAFNVAFEYIKRNVDKIDSFRARLMTAINTQEKFNELKSLLNEAIDEGILTQIQANET
IAAIEKNLKWQEKHLDDIKKWFXXXXXXXEEETTTTATVATTIETTTQGSNGKIISFYLV
CFSIILSYL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 27 | g9466.t1 | CDD | cd09601 | M1_APN-Q_like | 78 | 491 | 5.16973E-138 |
| 21 | g9466.t1 | Coils | Coil | Coil | 809 | 829 | - |
| 18 | g9466.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 61 | 278 | 1.5E-55 |
| 19 | g9466.t1 | Gene3D | G3DSA:1.10.390.60 | - | 279 | 493 | 4.0E-59 |
| 17 | g9466.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 494 | 553 | 6.0E-7 |
| 20 | g9466.t1 | Gene3D | G3DSA:1.25.50.20 | - | 555 | 868 | 6.2E-34 |
| 4 | g9466.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 49 | 490 | 7.8E-158 |
| 6 | g9466.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 49 | 490 | 7.8E-158 |
| 5 | g9466.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 517 | 872 | 7.8E-158 |
| 7 | g9466.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 517 | 872 | 7.8E-158 |
| 12 | g9466.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 211 | 226 | 1.1E-22 |
| 9 | g9466.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 260 | 275 | 1.1E-22 |
| 8 | g9466.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 339 | 349 | 1.1E-22 |
| 11 | g9466.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 378 | 393 | 1.1E-22 |
| 10 | g9466.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 397 | 409 | 1.1E-22 |
| 3 | g9466.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 76 | 270 | 2.2E-38 |
| 2 | g9466.t1 | Pfam | PF01433 | Peptidase family M1 domain | 306 | 490 | 2.0E-46 |
| 1 | g9466.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 538 | 848 | 3.8E-24 |
| 23 | g9466.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 24 | g9466.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 25 | g9466.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 11 | - |
| 26 | g9466.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 22 | g9466.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 909 | - |
| 14 | g9466.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 62 | 277 | 1.02E-41 |
| 13 | g9466.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 287 | 491 | 1.16E-46 |
| 16 | g9466.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 15 | g9466.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed