Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative DNA-directed RNA polymerase III subunit RPC3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g955 g955.t1 TSS g955.t1 7023540 7023540
chr_3 g955 g955.t1 isoform g955.t1 7024520 7025008
chr_3 g955 g955.t1 exon g955.t1.exon1 7024520 7025008
chr_3 g955 g955.t1 cds g955.t1.CDS1 7024520 7025008
chr_3 g955 g955.t1 TTS g955.t1 7025389 7025389

Sequences

>g955.t1 Gene=g955 Length=489
ATGTGGATTTTTGAAAAAAAAAAAGACGAAACTGGAAATGCAGTTTACAATGTTAATATG
AAGAAGGCCTTCGCACAAATGACTTGGTCAATAATCGATAATATCATCACACAGAAATTT
GGATCAAAAGCTGCGCGTATTTTTAGAGTTGTTCGCACTAAAAAGTTCATTGAACAGGAT
GAAATTCAACAAGAAGCAATGATACCACCAAAAGAGGCTCGATTGTTCACATATAAATTA
CTCGAAGAAAATCTTTTATTAATTAAATCGATCAAAAAGAGCGGAGGGATGGGACCATCA
AAACCAATTTACCTTTTTTATGTCAATCAACATCAAATTGCTCGTGATTTGATCGAGACT
TGTTATAAAACTCTTTACAATTTAACTGTTCGTGTAATTCAAGATAAAGAAATGAGTAAA
AGACTAATGGACAAAGAATTGCGACTTCAACTGGTCATTGACAATCTCAAAGAGCGCGGT
GAATCTTAA

>g955.t1 Gene=g955 Length=162
MWIFEKKKDETGNAVYNVNMKKAFAQMTWSIIDNIITQKFGSKAARIFRVVRTKKFIEQD
EIQQEAMIPPKEARLFTYKLLEENLLLIKSIKKSGGMGPSKPIYLFYVNQHQIARDLIET
CYKTLYNLTVRVIQDKEMSKRLMDKELRLQLVIDNLKERGES

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g955.t1 Gene3D G3DSA:1.10.10.10 winged helix repressor DNA binding domain 20 109 0
1 g955.t1 PANTHER PTHR12949 RNA POLYMERASE III DNA DIRECTED -RELATED 6 153 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003697 single-stranded DNA binding MF
GO:0005666 RNA polymerase III complex CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values