Gene loci information

Transcript annotation

  • This transcript has been annotated as Eukaryotic translation initiation factor 2-alpha kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g9617 g9617.t1 TTS g9617.t1 4300238 4300238
chr_1 g9617 g9617.t1 isoform g9617.t1 4300540 4304272
chr_1 g9617 g9617.t1 exon g9617.t1.exon1 4300540 4300722
chr_1 g9617 g9617.t1 cds g9617.t1.CDS1 4300540 4300722
chr_1 g9617 g9617.t1 exon g9617.t1.exon2 4300798 4301175
chr_1 g9617 g9617.t1 cds g9617.t1.CDS2 4300798 4301175
chr_1 g9617 g9617.t1 exon g9617.t1.exon3 4301236 4303768
chr_1 g9617 g9617.t1 cds g9617.t1.CDS3 4301236 4303768
chr_1 g9617 g9617.t1 exon g9617.t1.exon4 4304064 4304272
chr_1 g9617 g9617.t1 cds g9617.t1.CDS4 4304064 4304272
chr_1 g9617 g9617.t1 TSS g9617.t1 4304430 4304430

Sequences

>g9617.t1 Gene=g9617 Length=3303
ATGAAATTAATTTCGCTAGTAAATCGGCTACTTCCAGAATGTTCATACAAATTATTTGTG
CCGATAGTTTTTGGCATTTTATTATATGCAACAAAAAGTATTGCATCTGAGAACTTTGAT
GTTGATTATGTTGAAGGAGAAATAAGAACAGTAGAAGGAATATCGTTTTGTGATGAGAGC
CTTATAAAAGAGCGAGGATTGACAAAAAGGCTTCTTTTTATATCAACATTGGATGGTGGT
GTGACAGCATTAGATTTGACTAAAGATGGTCAAATTTTATGGGATATTTCAACAGGAGAA
CTGCTTTCATCTTCGATCCATCAAATGGAGATGACAAATCAAGGAAAATCTGTGCGAATG
ATACCGAGTTTAAATGGTGGCATTTATAAATTTGATGGCGAGTCGGTTGAAGCAATTCCA
GTGAATGCAGAAGATTTATTAAAAAGTTCATTCAAGTTCAAAGATGATATTGTAATGTCT
GGAAATCAAGAAGTAAAAACATACGGTGTAAATTGCAGAACAGGACAAGTTGTTTATGAG
CATCCGATTGGTGGCACAAGTAAAAAGAAAACAAATGATACATCTGATAAGGACTCAAAT
AAGAATGAAAATAGTCCAAGAACGACATCTGATTTAGTTCTTGATGATATTCTTGTAGTA
AGAAGATTTTCACAAACTGTCAGAGCAAATGAACCACGTAGTGGAGAAGAAAGGTGGAAT
TTTTCAATTGGCCATCATGAAATGCAAGTTGATCTAAGTGATAATTGTCACAAGAATGGT
GAAAGTGATATGATTGATAGAATGATACATGATACTGAAATTCGAGTTATAATTCCAGAA
GGTATCATTGTAGGATATAATAAGTACAATCCGAATGTAATTTTATGGAAACATAAATTT
AATTCACCAATCGTTAGCGTTTTCCGTTCTGATGAAAATAATCAATTGCATAATGTTGAT
CTTTTTGCTAATTCTCGCTGGCTTTGGGAAGAACATGGCAATCAATATTTTGTAAAAGCA
TCAGGTGATTCAAAATTTTCACCATCTCTTTATTTGGGTATTTACAATCAACAATATTAC
ATACAAGAATCCAATATGATGAAGTCTACCCTTGATAATATCAAAAAACAAGATCAAAAT
CTTGTTGAAGATGAAAGTAGACTTTTAAAAATTCCATTCAAACCCATTCCTGCCTCTAAT
AATGCACTTATTGATTTCGTTTCAAAGTTTAATACCAAAATTGAGAATATATCAAATGAT
AATGATGATGAAAAGAAAGAAATTAAAGATCTCATAAAAATTGATGATCAATTATTGACT
GCACAAGCCGTTTTTCTTGCACCTTCTCATGCTGAAGGGAGAGGCTTCTATTTGTTTTCA
TCAAAAGATATCAATGAAACAACACAATGCAAAAAGACAAAAAGTACTAATCAATCTCCT
CAAGATTTTGATAACATAACATTTCAAAATGAGAAAATACACCCAGTGAGACTTTCATTA
TGGTTTTATTGGAAAGAAATTTTAGTAATTGCACTTTCAACGGCTCTTATTATAAATATA
ATGTTAAGAAATCAAAATAGACGAGGTGAGAGAGAAGTTGTTTTTGTGCCTATTGCAAAG
GAGGCCATTGAATATGATGATGAAAGTGAAGAATTAAAGAAGAAGCATCTTGAAATAGAA
ATGCTTAGAAATGAAGCTCAAGCAAAATTAAGAAGTCTTTCTGAATCAGGTGAAAGAGAA
GAAAATTATAAATCACGCTTCCTTGAAGATTTTGATTTAGTGAGGTGTTTGGGAAAAGGA
GGTTTTGGTGTTGTATTTGAAGCAAGAAACAAGCTTGATGATTGTACATATGCAATCAAA
AGAATTATACTGCCAAGTAAACAAGAGTCACGTGAAAGAGTATTGCGTGAAGTTAAAACA
CTAGCCAATTGTGAGCATCACAATATTGTAAGATATTTCCATGCATGGGTTGAACAGCCG
CCAAAAGGATGGCAAGAATTGAAAGATAAAGAAATGTTTAGTCGCGATGTAATTTCAACT
TCAATTACAATCGACTCACCTTCACCAACTGAAGAATCAAAAGCATTCTCAACATACACT
GATAATAAATTGACATCATATGATTATAAAGCGAGCAATAATAAAACTGCAGCGTCCAGT
AATGATTGGCTGATGAACATTCAAAATAAAAGCTTTCCTGACTTCAGCGAAAATAATAAA
TTAATTGATGAATCAGAATTTGACGACTCGTGTATTGAATTCAAGGCAGATACAAGTGAC
GATGAAAACGATATTAAAGATAATTATAAAGACAATACCAGTATCGCTGATGAGTCATTT
GCTATTGAATTTAGAGAGTCTACTAGAACAGTATCATTATCTGATATGAATGGTGGTGAA
AGTGATGTAGAAAAAAGTCATGTGATTTCAATATCAGATGAAACGAGTATTTCTAAAAGT
ACTCAAAATGATCACAAAAAGAAGCATCGTCGTAATTTGTCGTTAGATCTAGCAACTAAT
GCAAATGACATGCTACCAAAGAAGTTGAATACAATTGGTGCCAATTCGAGCAAAATATAT
CTTTTTATTCAAATGCAATTATGTATGAAATCTAGTTTGAAAGATTGGCTTAAAGAGACA
GACTTGAAAGCAAGAAATGGAGAGACTGTTAATATTTGGCATCAAATAATAGATGCTGTA
CATTATGTACATCTTAAAGGACTTATTCATCGAGATTTAAAGCCCAGTAACATTTTCTTT
GCTCTTGATGGTAAAATCAAAATTGGTGATTTTGGTCTAGTTACGGATATGGCAGAAATA
CCTTTTCCTGATCCTCTCACATCTTCATCTTCAAATTCAAATTCATCTTCAAACTTTGAT
GCAGATTTTGTTTTCATTGGTAGCAAAAAGCATACACAACGTGTTGGTACTTCACTCTAT
ATGAGTCCTGAGCAAGCTAGAGGAGGAGCCTACAATTATAAAGTTGATATTTACTCATTA
GGTCTTATTTTCTTTGAACTGCTTAACTTTTTCTATACTGAGACTGAACGATATAAAGTT
CTACAAGATATAAAAAATCAAAAATATCCCAATTCATTCATAAATGAATTTAAGAATGAA
TTCGAGCTACTTAAAATGATGCTTGCAAAGTGCCCAGAGGAAAGGCCGACAACATTTGGG
ATTCGAGCAAGACCACCTTTAAATAAAGTTGATAATGAACTCAGTTCAACTGAGTGGCAT
TTTGAATTGCCAACAAGAAGAAGAGATAGTTTTCGTTCAATTAATAATTTGAATGGAAAG
TAA

>g9617.t1 Gene=g9617 Length=1100
MKLISLVNRLLPECSYKLFVPIVFGILLYATKSIASENFDVDYVEGEIRTVEGISFCDES
LIKERGLTKRLLFISTLDGGVTALDLTKDGQILWDISTGELLSSSIHQMEMTNQGKSVRM
IPSLNGGIYKFDGESVEAIPVNAEDLLKSSFKFKDDIVMSGNQEVKTYGVNCRTGQVVYE
HPIGGTSKKKTNDTSDKDSNKNENSPRTTSDLVLDDILVVRRFSQTVRANEPRSGEERWN
FSIGHHEMQVDLSDNCHKNGESDMIDRMIHDTEIRVIIPEGIIVGYNKYNPNVILWKHKF
NSPIVSVFRSDENNQLHNVDLFANSRWLWEEHGNQYFVKASGDSKFSPSLYLGIYNQQYY
IQESNMMKSTLDNIKKQDQNLVEDESRLLKIPFKPIPASNNALIDFVSKFNTKIENISND
NDDEKKEIKDLIKIDDQLLTAQAVFLAPSHAEGRGFYLFSSKDINETTQCKKTKSTNQSP
QDFDNITFQNEKIHPVRLSLWFYWKEILVIALSTALIINIMLRNQNRRGEREVVFVPIAK
EAIEYDDESEELKKKHLEIEMLRNEAQAKLRSLSESGEREENYKSRFLEDFDLVRCLGKG
GFGVVFEARNKLDDCTYAIKRIILPSKQESRERVLREVKTLANCEHHNIVRYFHAWVEQP
PKGWQELKDKEMFSRDVISTSITIDSPSPTEESKAFSTYTDNKLTSYDYKASNNKTAASS
NDWLMNIQNKSFPDFSENNKLIDESEFDDSCIEFKADTSDDENDIKDNYKDNTSIADESF
AIEFRESTRTVSLSDMNGGESDVEKSHVISISDETSISKSTQNDHKKKHRRNLSLDLATN
ANDMLPKKLNTIGANSSKIYLFIQMQLCMKSSLKDWLKETDLKARNGETVNIWHQIIDAV
HYVHLKGLIHRDLKPSNIFFALDGKIKIGDFGLVTDMAEIPFPDPLTSSSSNSNSSSNFD
ADFVFIGSKKHTQRVGTSLYMSPEQARGGAYNYKVDIYSLGLIFFELLNFFYTETERYKV
LQDIKNQKYPNSFINEFKNEFELLKMMLAKCPEERPTTFGIRARPPLNKVDNELSSTEWH
FELPTRRRDSFRSINNLNGK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g9617.t1 Coils Coil Coil 407 434 -
11 g9617.t1 Coils Coil Coil 545 569 -
7 g9617.t1 Gene3D G3DSA:2.130.10.10 - 66 363 1.3E-9
8 g9617.t1 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 584 677 9.9E-33
9 g9617.t1 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 868 1077 3.6E-46
19 g9617.t1 MobiDBLite mobidb-lite consensus disorder prediction 181 208 -
18 g9617.t1 MobiDBLite mobidb-lite consensus disorder prediction 186 204 -
3 g9617.t1 PANTHER PTHR11042:SF91 EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 3 452 1058 1.8E-94
4 g9617.t1 PANTHER PTHR11042 EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED 452 1058 1.8E-94
1 g9617.t1 Pfam PF00069 Protein kinase domain 593 658 3.4E-12
2 g9617.t1 Pfam PF00069 Protein kinase domain 831 1058 1.9E-32
13 g9617.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 500 -
14 g9617.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 501 522 -
12 g9617.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 523 1100 -
17 g9617.t1 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 597 620 -
16 g9617.t1 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 908 920 -
20 g9617.t1 ProSiteProfiles PS50011 Protein kinase domain profile. 591 1067 32.23
15 g9617.t1 SMART SM00220 serkin_6 591 1067 5.8E-39
6 g9617.t1 SUPERFAMILY SSF50998 Quinoprotein alcohol dehydrogenase-like 70 249 6.15E-11
5 g9617.t1 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 586 1086 6.38E-62

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0005515 protein binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values