Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Eukaryotic translation initiation factor 2-alpha kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g9617 g9617.t2 isoform g9617.t2 4299910 4301345
chr_1 g9617 g9617.t2 exon g9617.t2.exon1 4299910 4300722
chr_1 g9617 g9617.t2 cds g9617.t2.CDS1 4300540 4300722
chr_1 g9617 g9617.t2 exon g9617.t2.exon2 4300798 4301175
chr_1 g9617 g9617.t2 cds g9617.t2.CDS2 4300798 4301109
chr_1 g9617 g9617.t2 exon g9617.t2.exon3 4301236 4301345
chr_1 g9617 g9617.t2 TSS g9617.t2 NA NA
chr_1 g9617 g9617.t2 TTS g9617.t2 NA NA

Sequences

>g9617.t2 Gene=g9617 Length=1301
AAGAGACAGACTTGAAAGCAAGAAATGGAGAGACTGTTAATATTTGGCATCAAATAATAG
ATGCTGTACATTATGTACATCTTAAAGGACTTATTCATCGAGATTTAAAGCCCAGTAACA
TTTTCTTTGCTCTTGATGGTAAAATCAAAATTGGTGATTTTGGTCTAGTTACGGATATGG
CAGAAATACCTTTTCCTGATCCTCTCACATCTTCATCTTCAAATTCAAATTCATCTTCAA
ACTTTGATGCAGATTTTGTTTTCATTGGTAGCAAAAAGCATACACAACGTGTTGGTACTT
CACTCTATATGAGTCCTGAGCAAGCTAGAGGAGGAGCCTACAATTATAAAGTTGATATTT
ACTCATTAGGTCTTATTTTCTTTGAACTGCTTAACTTTTTCTATACTGAGACTGAACGAT
ATAAAGTTCTACAAGATATAAAAAATCAAAAATATCCCAATTCATTCATAAATGAATTTA
AGAATGAATTCGAGCTACTTAAAATGATGCTTGCAAAGTGCCCAGAGGAAAGGCCGACAA
CATTTGGGATTCGAGCAAGACCACCTTTAAATAAAGTTGATAATGAACTCAGTTCAACTG
AGTGGCATTTTGAATTGCCAACAAGAAGAAGAGATAGTTTTCGTTCAATTAATAATTTGA
ATGGAAAGTAAGCTATAATTTTACTATGCGTTATTAAGTAAGATTCTTTTGCAATTTGCA
ACCCCTTTATTTTAATTTTTAGTTTTTACTAAAATTTCTGTTCTAAGCTCAATGAGTATT
TTTCCCTTTTAATTGTTTGAAACATATTTTTACCTCAAATATGATTGGTATGTTACAAAG
TTAATTCTATTTCCTTATGGAAAAGTGCATGTGATTTATTAATTTTAATAAAATGATATC
CTAAAATTATTAATGCAAAGTGTGGTATACTTTGCAATAAATAAAAATTCAAAAAGAAAA
TGATATCAATAATAAAAAATTTATTTTTACTTATTATACAAACCTCGATGCGAATAGTTA
AGGAAATATTTTGATAAGCAATTACTTGAATCAATATTGCCGAAATAAAATCTTTATTAA
AGGTCAATTTAAAATTCATTCTTTAATTTAATTAACATATTATTTCAGTTTCGCAATGTA
CATATTGCCTTTAAAAAAATGACATCACAACATCCGTTCTTTGTTAAATAACACTTATTT
CACTTCTATAATAAATTCTAATACTTTTATTTCTCATGAATAAAAAGATAGATGTTTATT
AAATACAAAATAATTTAGTTTATTTCAAACGAAAAAAATTC

>g9617.t2 Gene=g9617 Length=164
MAEIPFPDPLTSSSSNSNSSSNFDADFVFIGSKKHTQRVGTSLYMSPEQARGGAYNYKVD
IYSLGLIFFELLNFFYTETERYKVLQDIKNQKYPNSFINEFKNEFELLKMMLAKCPEERP
TTFGIRARPPLNKVDNELSSTEWHFELPTRRRDSFRSINNLNGK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g9617.t2 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 6 142 0.000
2 g9617.t2 PANTHER PTHR11042 EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED 33 126 0.000
3 g9617.t2 PANTHER PTHR11042:SF163 INTERFERON-INDUCED, DOUBLE-STRANDED RNA-ACTIVATED PROTEIN KINASE 33 126 0.000
1 g9617.t2 Pfam PF00069 Protein kinase domain 31 123 0.000
6 g9617.t2 ProSiteProfiles PS50011 Protein kinase domain profile. 1 131 12.598
4 g9617.t2 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 34 134 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values