General information for g9654.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein kinase C.
Parent gene
- The parent gene g9654 is located on chr_1: 4487667..4491874 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9654 | g9654.t1 | TSS | g9654.t1 | 4487327 | 4487327 |
| chr_1 | g9654 | g9654.t1 | isoform | g9654.t1 | 4487667 | 4491874 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon1 | 4487667 | 4487820 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS1 | 4487667 | 4487820 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon2 | 4488629 | 4488880 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS2 | 4488629 | 4488880 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon3 | 4488946 | 4489117 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS3 | 4488946 | 4489117 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon4 | 4489407 | 4489414 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS4 | 4489407 | 4489414 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon5 | 4489603 | 4489626 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS5 | 4489603 | 4489626 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon6 | 4489922 | 4490108 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS6 | 4489922 | 4490108 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon7 | 4490175 | 4490796 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS7 | 4490175 | 4490796 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon8 | 4490861 | 4491122 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS8 | 4490861 | 4491122 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon9 | 4491183 | 4491377 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS9 | 4491183 | 4491377 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon10 | 4491438 | 4491518 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS10 | 4491438 | 4491518 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon11 | 4491579 | 4491637 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS11 | 4491579 | 4491637 |
| chr_1 | g9654 | g9654.t1 | exon | g9654.t1.exon12 | 4491707 | 4491874 |
| chr_1 | g9654 | g9654.t1 | cds | g9654.t1.CDS12 | 4491707 | 4491874 |
| chr_1 | g9654 | g9654.t1 | TTS | g9654.t1 | NA | NA |
Sequences
>g9654.t1 Gene=g9654 Length=2184
ATGTTTACGGGTACATTGAGGGTAAAAATATGCGAAGCAAAGGATTTACGACCAACAGAC
TATCAGAAAAGACACGAATTGAATTTTGGAAAGAACAACGATAAGAAAGATCTTGATCCT
TATGTATCATTAAATGTGGATGATAAATTTATAGATCGTTCAACTATCAAACAAAAAACA
TTTGATCCTGTATGGAATGAGACTTATATTCATGAAGTGGAAAATGCAAACAGCTTGGGA
ATAACAGTTTTTCATAAAGCACCAATTGATGATGTTTTTAATGCTAATGCAACAATATCG
TTTGAGGATTTGATAGCACGTAATCAACAAGAGCAGACTGACTTTTGGGTTGACTTGGAA
CCATGTGGTCGGTTACATGTAAAAATCGATCTACGATGGTCGAAAGAAACAAGTCAAAAC
CAAGCGATTCCTATGCGTCGAACAAACAAAGAACAGCCGTTTTTGAATCGTCGTCGTGGT
GCGATGAGACGACGTGTTCATCAAGTAAATGGTCACAAGTTTATGGCAACATTTCTACGA
CAGCCAACATTTTGTTCTCATTGTCGTGAATTCATTTGGGGTCTAGGAAAACAAGGCTAT
CAATGCCAAGTATGCACTTGTGTTGTTCACAAACGATGTCATCAATTGGTCGTGACAAAA
TGTCCGAGGAAAAAGGATGAAAAGTTGAAAGAGAATGAGGAAGTCGTCAATAGTCAACGC
TTTAACGTAAACATTCCTCATCGCTTTGCAGTGCATAGTTTCAAACGTTTGACATTTTGT
GATCATTGTGGTAGCTTATTGTATGGAATAATTCGACAAGGATTAAAATGTGAAGTATGC
AGTATGAATATTCATCGTCGATGTGAAGGAAATGTTGCAAATAATTGTGGCATCAATACT
AAACAGTTAGCCGAATTATTAAATGAAATGGGGATTACAATGGACAAATCAACACCACCG
CGTCGCTCCAAATATATCAACCAAGCAGCTAGCGATACATCTTCAACAAGTGATCGTAGC
GGGGATGATGCTGTGTCACTTGATACAATGTCAACGGCATCAACACGTACAACAACATCA
GATATATCAAAAGCTGGTACAAGTGCTAGTCGTCAGTTATTACAGCAATCTGATGAAAGT
GGAATTACTCAAATTTCTGGAAAACTTACGCTCACTGATTTTAATTTCATCAAAGTATTG
GGTAAGGGATCATTTGGAAAAGTCATGCTAGCAGAACGTAAAGGCTCAGAAGAAATTTAT
GCCGTAAAAGTATTGAAGAAAGATGCCATCTTACAAGATGACGATGTCGACTGCACAATG
ACTGAAAAGCGAATTTTAACGCTTGCTGCTCGTCATCCATTTTTGACTGCATTACATTCT
TGCTTTCAAACACCTGATAGATTATTCTTTGTCATGGAGTATGTTAACGGTGGCGATTTA
ATGTTCCAAATTCAACGTGCTCGAAAGTTTGATGAAGCACGAGCTGCTTTTTATGCAGCT
GAAGTTACTTTAGCTCTTCAATTCTTACATCGTAATGGAGTTATATATCGTGATTTAAAA
TTGGATAATATTCTATTAGATGCTGAAGGTCATTGCAAGTTGGCTGATTTTGGTATGTGC
AAAGAAGGAATCATGGGTGGGAATTTGACATCAACATTTTGTGGTACACCAGATTATATA
GCTCCTGAGATTTTGCAAGAATTAGATTATGGTCCATCAGTTGATTGGTGGGCTTTAGGA
GTTTTGATGTATGAAATGATGGCTGGACAACCTCCGTTTGAAGCCGATAATGAAGATGAC
TTATTTGAAGCTATACTTCGTGATGACGTTTTATATCCTGTATGGTTATCACGTGAAGCT
GTTTCTATATTAAAAGGTTTCATGACGAAAAATCCAGCTCGTCGATTAGGATGTATCGAT
GGTGAAGATTCAATCAGAAAGCATCCATTTTTCAAAGATATGGATTGGGATTTGTTAGAG
CAACGTAAAGTTCGTCCACCATTTAGGCCAAAAGTTAGAAGCGCACGTGATGCAGTCAAT
TTTGACACAGAATTTACAAGAGAAGAACCGGTCTTAACACCAGTTCCAGCTGATATAATT
CGTTGCATTAATCAAGACGAATTTGCTGGATTTTCGTTTGTTAACAAAGAATTTGGACCG
GAGAGAAAAAGTTTAGGCAGTTAA
>g9654.t1 Gene=g9654 Length=727
MFTGTLRVKICEAKDLRPTDYQKRHELNFGKNNDKKDLDPYVSLNVDDKFIDRSTIKQKT
FDPVWNETYIHEVENANSLGITVFHKAPIDDVFNANATISFEDLIARNQQEQTDFWVDLE
PCGRLHVKIDLRWSKETSQNQAIPMRRTNKEQPFLNRRRGAMRRRVHQVNGHKFMATFLR
QPTFCSHCREFIWGLGKQGYQCQVCTCVVHKRCHQLVVTKCPRKKDEKLKENEEVVNSQR
FNVNIPHRFAVHSFKRLTFCDHCGSLLYGIIRQGLKCEVCSMNIHRRCEGNVANNCGINT
KQLAELLNEMGITMDKSTPPRRSKYINQAASDTSSTSDRSGDDAVSLDTMSTASTRTTTS
DISKAGTSASRQLLQQSDESGITQISGKLTLTDFNFIKVLGKGSFGKVMLAERKGSEEIY
AVKVLKKDAILQDDDVDCTMTEKRILTLAARHPFLTALHSCFQTPDRLFFVMEYVNGGDL
MFQIQRARKFDEARAAFYAAEVTLALQFLHRNGVIYRDLKLDNILLDAEGHCKLADFGMC
KEGIMGGNLTSTFCGTPDYIAPEILQELDYGPSVDWWALGVLMYEMMAGQPPFEADNEDD
LFEAILRDDVLYPVWLSREAVSILKGFMTKNPARRLGCIDGEDSIRKHPFFKDMDWDLLE
QRKVRPPFRPKVRSARDAVNFDTEFTREEPVLTPVPADIIRCINQDEFAGFSFVNKEFGP
ERKSLGS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g9654.t1 | CDD | cd04014 | C2_PKC_epsilon | 1 | 134 | 6.72739E-62 |
| 24 | g9654.t1 | CDD | cd00029 | C1 | 172 | 221 | 9.39352E-15 |
| 23 | g9654.t1 | CDD | cd00029 | C1 | 247 | 296 | 2.08965E-15 |
| 25 | g9654.t1 | CDD | cd05591 | STKc_nPKC_epsilon | 398 | 715 | 0.0 |
| 20 | g9654.t1 | Gene3D | G3DSA:2.60.40.150 | - | 1 | 132 | 4.0E-29 |
| 18 | g9654.t1 | Gene3D | G3DSA:3.30.60.20 | - | 159 | 237 | 1.0E-23 |
| 17 | g9654.t1 | Gene3D | G3DSA:3.30.60.20 | - | 243 | 308 | 6.6E-22 |
| 16 | g9654.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 392 | 708 | 3.4E-140 |
| 19 | g9654.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 478 | 682 | 3.4E-140 |
| 34 | g9654.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 327 | 370 | - |
| 6 | g9654.t1 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 6 | 719 | 0.0 |
| 7 | g9654.t1 | PANTHER | PTHR24356:SF240 | PROTEIN KINASE C | 6 | 719 | 0.0 |
| 21 | g9654.t1 | PIRSF | PIRSF000551 | PKC_delta | 1 | 723 | 0.0 |
| 11 | g9654.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 169 | 183 | 4.7E-13 |
| 8 | g9654.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 185 | 194 | 4.7E-13 |
| 9 | g9654.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 198 | 209 | 4.7E-13 |
| 10 | g9654.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 285 | 297 | 4.7E-13 |
| 2 | g9654.t1 | Pfam | PF00168 | C2 domain | 4 | 116 | 1.9E-14 |
| 5 | g9654.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 172 | 223 | 1.0E-16 |
| 4 | g9654.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 247 | 298 | 6.1E-15 |
| 3 | g9654.t1 | Pfam | PF00069 | Protein kinase domain | 395 | 651 | 2.5E-64 |
| 1 | g9654.t1 | Pfam | PF00433 | Protein kinase C terminal domain | 672 | 713 | 1.4E-7 |
| 32 | g9654.t1 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 247 | 296 | - |
| 33 | g9654.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 400 | 423 | - |
| 31 | g9654.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 514 | 526 | - |
| 35 | g9654.t1 | ProSiteProfiles | PS50004 | C2 domain profile. | 1 | 109 | 12.321 |
| 37 | g9654.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 171 | 221 | 16.1 |
| 38 | g9654.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 246 | 296 | 15.673 |
| 39 | g9654.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 394 | 651 | 48.725 |
| 36 | g9654.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 652 | 723 | 18.0 |
| 26 | g9654.t1 | SMART | SM00239 | C2_3c | 5 | 113 | 4.9E-13 |
| 30 | g9654.t1 | SMART | SM00109 | c1_12 | 172 | 221 | 4.9E-14 |
| 29 | g9654.t1 | SMART | SM00109 | c1_12 | 247 | 296 | 1.3E-14 |
| 28 | g9654.t1 | SMART | SM00220 | serkin_6 | 394 | 651 | 3.7E-101 |
| 27 | g9654.t1 | SMART | SM00133 | pkinase_C_6 | 652 | 715 | 4.3E-25 |
| 15 | g9654.t1 | SUPERFAMILY | SSF49562 | C2 domain (Calcium/lipid-binding domain, CaLB) | 2 | 131 | 9.97E-26 |
| 12 | g9654.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 157 | 223 | 3.29E-20 |
| 13 | g9654.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 238 | 301 | 4.34E-20 |
| 14 | g9654.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 390 | 714 | 9.85E-93 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
| GO:0004697 | protein kinase C activity | MF |
KEGG
Orthology
- This transcript has been annotated with K18050 (novel protein kinase C epsilon type)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
