Gene loci information

Transcript annotation

  • This transcript has been annotated as 5-aminolevulinate synthase, erythroid-specific, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g9675 g9675.t8 TTS g9675.t8 4537617 4537617
chr_1 g9675 g9675.t8 isoform g9675.t8 4538494 4539742
chr_1 g9675 g9675.t8 exon g9675.t8.exon1 4538494 4539536
chr_1 g9675 g9675.t8 cds g9675.t8.CDS1 4538496 4539536
chr_1 g9675 g9675.t8 exon g9675.t8.exon2 4539740 4539742
chr_1 g9675 g9675.t8 cds g9675.t8.CDS2 4539740 4539742
chr_1 g9675 g9675.t8 TSS g9675.t8 4539997 4539997

Sequences

>g9675.t8 Gene=g9675 Length=1046
ATGAATCAACTTGTTAAATCAACTTCAAGCCTAATCCGCACGGTGAGCAGCAAAATGCCT
TGCCCATTTTTGACGCGTTTGAGTTCAACTTATGTCCGCAATTATGCACCAATCCTTATG
AAGACCTACGGCAATCAATGCCCTGTCGTTGCTCGTGCAATTTCTCAATTGCAAAATGCC
GCTGGTGCTACAACTATCCAAAATCAAGACCGTAAATTGAGCTCAACTCAAACTGAACCA
ATTGCTGAGAATCAATGCCAAGCGCCACCATCATTGGCTGAAGTTGATTCGCATTTGAAA
GAAGTTAATGAAGCTCTTTTGCAAGATATTCCACAAAATCGCGAATCACAACCACTTTTT
GCTTATGAAGATTTCTTTCATGAACAAATCATGAAGAAAAAGCGTGATCATTCATATCGT
GTGTTCAAGAAAGTCAATCGTTTAGCTGGTCCTGGACAATTCCCAAAAGGTCTCGAATAT
GGCAATCGTCCAATTACTGTTTGGTGTTCAAACGACTACTTAGGCATGTCATCGCATCCT
GAAGTTAAAAATGCCGTGCATGAAGCCTTAGAAAAATACGGCGCTGGTGCTGGAGGTACC
CGTAACATTTCTGGAAATTCCATGATGCATGAAGCACTTGAAAAGCAATTGGCTGGCTTA
CATCAAAAGGAAGCAGCACTTGTCTTCTCATCATGCTATGTGGCAAATGATTCAACTCTT
TTCACTCTCGCTAAATTGCTACCTGGTTGTCATATCTTTTCTGATGCTGGAAATCACGCT
TCAATGATTCAAGGCATAAGAAATAGTGGTGTGCCAAAGCACATCTTTCGGCATAATGAT
CCTGATCATTTACGCGAACTTATCTCGAAAATTCCGAAAGAAGTGCCAAAAATTGTTGCT
TTCGAGACTGTGCATAGCATGACTGGCGCTGTCTGCCCTTTGGAAGAACTCTGTGATATC
GCTCATGAACATGGTGCTATTACATTTGTCGATGAAGTTCATGCCGTAGGTCTCTATGGT
GAACATGGTGCTGGAATTGGTGAACG

>g9675.t8 Gene=g9675 Length=348
MNQLVKSTSSLIRTVSSKMPCPFLTRLSSTYVRNYAPILMKTYGNQCPVVARAISQLQNA
AGATTIQNQDRKLSSTQTEPIAENQCQAPPSLAEVDSHLKEVNEALLQDIPQNRESQPLF
AYEDFFHEQIMKKKRDHSYRVFKKVNRLAGPGQFPKGLEYGNRPITVWCSNDYLGMSSHP
EVKNAVHEALEKYGAGAGGTRNISGNSMMHEALEKQLAGLHQKEAALVFSSCYVANDSTL
FTLAKLLPGCHIFSDAGNHASMIQGIRNSGVPKHIFRHNDPDHLRELISKIPKEVPKIVA
FETVHSMTGAVCPLEELCDIAHEHGAITFVDEVHAVGLYGEHGAGIGE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g9675.t8 Gene3D G3DSA:3.40.640.10 - 180 348 7.3E-62
2 g9675.t8 PANTHER PTHR13693 CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE 64 348 1.4E-124
3 g9675.t8 PANTHER PTHR13693:SF58 5-AMINOLEVULINATE SYNTHASE, ERYTHROID-SPECIFIC, MITOCHONDRIAL 64 348 1.4E-124
1 g9675.t8 Pfam PF00155 Aminotransferase class I and II 165 343 7.8E-47
4 g9675.t8 SUPERFAMILY SSF53383 PLP-dependent transferases 120 346 1.43E-63
5 g9675.t8 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM 1 18 -
7 g9675.t8 TIGRFAM TIGR01821 5aminolev_synth: 5-aminolevulinic acid synthase 121 348 3.2E-106

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0009058 biosynthetic process BP
GO:0033014 tetrapyrrole biosynthetic process BP
GO:0003870 5-aminolevulinate synthase activity MF
GO:0003824 catalytic activity MF
GO:0030170 pyridoxal phosphate binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed