General information for g9730.t38
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Purine nucleoside phosphorylase.
Parent gene
- The parent gene g9730 is located on chr_1: 4814085..4823379 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9730 | g9730.t38 | TTS | g9730.t38 | 4813145 | 4813145 |
| chr_1 | g9730 | g9730.t38 | isoform | g9730.t38 | 4813302 | 4815255 |
| chr_1 | g9730 | g9730.t38 | exon | g9730.t38.exon1 | 4813302 | 4813414 |
| chr_1 | g9730 | g9730.t38 | exon | g9730.t38.exon2 | 4813649 | 4813916 |
| chr_1 | g9730 | g9730.t38 | cds | g9730.t38.CDS1 | 4813679 | 4813916 |
| chr_1 | g9730 | g9730.t38 | exon | g9730.t38.exon3 | 4813976 | 4814425 |
| chr_1 | g9730 | g9730.t38 | cds | g9730.t38.CDS2 | 4813976 | 4814425 |
| chr_1 | g9730 | g9730.t38 | exon | g9730.t38.exon4 | 4815134 | 4815255 |
| chr_1 | g9730 | g9730.t38 | cds | g9730.t38.CDS3 | 4815134 | 4815255 |
| chr_1 | g9730 | g9730.t38 | TSS | g9730.t38 | 4815341 | 4815341 |
Sequences
>g9730.t38 Gene=g9730 Length=953
ATGCCAGAAGAAATAATTCCAGGATATTTTAATGGAAATGTAGTTAACAGCAAAGTTGCA
GGCAATTCATCTCCCATACCACCGAGAACTCCTTCACCAACTACTTCAACATCCCTGGAT
ATTTACACATACGAAACAATCATCGAGATAGCAAACTTCCTTCTACAAAGAACAAGCATT
CGCCCAAAAATTGGTATCATTTGCGGTTCTGGTCTTGGACATTTGGCAGAAAATTTGACT
GAAGCTGATTCATTTCCATATGAAGAAATTCCAAATTTTCCCGTCTCAACAGTTGCAGGA
CATGCAGGCCGAATGGTATTTGGTTATTTGAATGGCATTGAAGTGATGTGTATGCAAGGA
AGATTTCATTATTATGAAGGATATGCTTTAAGTACATGCTCAATGCCTGTAAGAGTCATG
AAATTCTGCGGATGCACACACTTGATTGCTACAAATGCTGCTGGTGGTTTAAACAGTTCA
TATAATGTTGGTGACATCATGATTGTTAAAGATCACATAAATATTATGGGATTTGCAGGA
AATTCACCTTTGCAAGGACCTAATGATCATAGATTTGGACCACGATTTCCACCGATGAAT
AAAGCTTACGATCCTGAATTAATCGAAATTGCACAAAATATTTCTAAAGAGATGGGAATG
GAAAATGACACTCACACAGGTGTATACATTTGTCTAGGTGGACCAAGTTATGAAACAGTC
GCTGAATTAAAAATGTGGAGTATTCTAGGTGTTGATGCTGTCGGCATGTCAACTGTAAGT
TATTATCTCATTAGAATATTATTTTCATAATTAAATTATCATTCATTCTAGGTTCATGAA
CTAATCATGAGGAGGTTATAACTACAGGTTCTAAGAGACAAAATGTGTTGACAAACTTTG
TTACTCGTATGGTAGAAAATATAAACAGTCTAATCATTAACGATTCAATGTAA
>g9730.t38 Gene=g9730 Length=269
MPEEIIPGYFNGNVVNSKVAGNSSPIPPRTPSPTTSTSLDIYTYETIIEIANFLLQRTSI
RPKIGIICGSGLGHLAENLTEADSFPYEEIPNFPVSTVAGHAGRMVFGYLNGIEVMCMQG
RFHYYEGYALSTCSMPVRVMKFCGCTHLIATNAAGGLNSSYNVGDIMIVKDHINIMGFAG
NSPLQGPNDHRFGPRFPPMNKAYDPELIEIAQNISKEMGMENDTHTGVYICLGGPSYETV
AELKMWSILGVDAVGMSTVSYYLIRILFS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g9730.t38 | CDD | cd09009 | PNP-EcPNPII_like | 45 | 259 | 3.42933E-128 |
| 4 | g9730.t38 | Gene3D | G3DSA:3.40.50.1580 | - | 42 | 264 | 1.6E-97 |
| 2 | g9730.t38 | PANTHER | PTHR11904 | METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE | 42 | 260 | 2.0E-94 |
| 8 | g9730.t38 | PIRSF | PIRSF000477 | PurNPase | 37 | 264 | 4.1E-86 |
| 1 | g9730.t38 | Pfam | PF01048 | Phosphorylase superfamily | 63 | 260 | 2.1E-38 |
| 6 | g9730.t38 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 244 | - |
| 7 | g9730.t38 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 245 | 264 | - |
| 5 | g9730.t38 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 265 | 269 | - |
| 3 | g9730.t38 | SUPERFAMILY | SSF53167 | Purine and uridine phosphorylases | 41 | 260 | 4.32E-80 |
| 10 | g9730.t38 | TIGRFAM | TIGR01697 | PNPH-PUNA-XAPA: inosine/guanosine/xanthosine phosphorylase family | 63 | 260 | 1.9E-81 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004731 | purine-nucleoside phosphorylase activity | MF |
| GO:0009116 | nucleoside metabolic process | BP |
| GO:0006139 | nucleobase-containing compound metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03783 (purine-nucleoside phosphorylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
