Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Purine nucleoside phosphorylase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g9730 g9730.t55 TTS g9730.t55 4813145 4813145
chr_1 g9730 g9730.t55 isoform g9730.t55 4813745 4814435
chr_1 g9730 g9730.t55 exon g9730.t55.exon1 4813745 4813916
chr_1 g9730 g9730.t55 cds g9730.t55.CDS1 4813745 4813916
chr_1 g9730 g9730.t55 exon g9730.t55.exon2 4813976 4814435
chr_1 g9730 g9730.t55 cds g9730.t55.CDS2 4813976 4814235
chr_1 g9730 g9730.t55 TSS g9730.t55 4815341 4815341

Sequences

>g9730.t55 Gene=g9730 Length=632
CCATTCACAGTTACACATACGAAACAATCATCGAGATAGCAAACTTCCTTCTACAAAGAA
CAAGCATTCGCCCAAAAATTGGTATCATTTGCGGTTCTGGTCTTGGACATTTGGCAGAAA
ATTTGACTGAAGCTGATTCATTTCCATATGAAGAAATTCCAAATTTTCCCGTCTCAACAG
TTGCAGGACATGCAGGCCGAATGGTATTTGGTTATTTGAATGGCATTGAAGTGATGTGTA
TGCAAGGAAGATTTCATTATTATGAAGGATATGCTTTAAGTACATGCTCAATGCCTGTAA
GAGTCATGAAATTCTGCGGATGCACACACTTGATTGCTACAAATGCTGCTGGTGGTTTAA
ACAGTTCATATAATGTTGGTGACATCATGATTGTTAAAGATCACATAAATATTATGGGAT
TTGCAGGAAATTCACCTTTGCAAGGACCTAATGATCATAGATTTGGACCACGATTTCCAC
CGATGAATAAAGCTTACGATCCTGAATTAATCGAAATTGCACAAAATATTTCTAAAGAGA
TGGGAATGGAAAATGACACTCACACAGGTGTATACATTTGTCTAGGTGGACCAAGTTATG
AAACAGTCGCTGAATTAAAAATGTGGAGTATT

>g9730.t55 Gene=g9730 Length=144
MVFGYLNGIEVMCMQGRFHYYEGYALSTCSMPVRVMKFCGCTHLIATNAAGGLNSSYNVG
DIMIVKDHINIMGFAGNSPLQGPNDHRFGPRFPPMNKAYDPELIEIAQNISKEMGMENDT
HTGVYICLGGPSYETVAELKMWSI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g9730.t55 CDD cd09009 PNP-EcPNPII_like 1 141 0
4 g9730.t55 Gene3D G3DSA:3.40.50.1580 - 1 144 0
2 g9730.t55 PANTHER PTHR11904 METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE 1 142 0
1 g9730.t55 Pfam PF01048 Phosphorylase superfamily 2 143 0
3 g9730.t55 SUPERFAMILY SSF53167 Purine and uridine phosphorylases 2 143 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004731 purine-nucleoside phosphorylase activity MF
GO:0009116 nucleoside metabolic process BP
GO:0006139 nucleobase-containing compound metabolic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values