General information for g9731.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Purine nucleoside phosphorylase.
Parent gene
- The parent gene g9731 is located on chr_1: 4816850..4817866 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9731 | g9731.t2 | isoform | g9731.t2 | 4816850 | 4817866 |
| chr_1 | g9731 | g9731.t2 | exon | g9731.t2.exon1 | 4816850 | 4817034 |
| chr_1 | g9731 | g9731.t2 | cds | g9731.t2.CDS1 | 4816851 | 4817034 |
| chr_1 | g9731 | g9731.t2 | exon | g9731.t2.exon2 | 4817094 | 4817543 |
| chr_1 | g9731 | g9731.t2 | cds | g9731.t2.CDS2 | 4817094 | 4817543 |
| chr_1 | g9731 | g9731.t2 | exon | g9731.t2.exon3 | 4817730 | 4817866 |
| chr_1 | g9731 | g9731.t2 | cds | g9731.t2.CDS3 | 4817730 | 4817866 |
| chr_1 | g9731 | g9731.t2 | TSS | g9731.t2 | 4817996 | 4817996 |
| chr_1 | g9731 | g9731.t2 | TTS | g9731.t2 | NA | NA |
Sequences
>g9731.t2 Gene=g9731 Length=772
ATGTCACAGATCATTATCAATGGAAATGGCAATCATTATGACGATAGTCATAAAATTAAA
AATGGCAATTCATATAAAAATAATAATTCGTCTCCTCCAAGAACAGCTTCACCAATCTTC
TCTTATGTCAACGAAAATTACTCGTATGAGATAATTGATAATATCAAAAATTATCTAATG
GCACGAACAAAAATACGCCCAAAAATTGGTATCATTTGCGGCACTGGTCTTGGACATTTG
GCTGAAAATTTGACTGAAGCTGATTCATTTCCATATGAAGAAATTCCAAATTTTCCCGTC
TCAACAGTTGCAGGACATGCAGGCCGAATGGTATTTGGTTATTTGAATGGCATTGAAGTA
ATGTGTATGCAAGGGAGATTTCATTATTATGAAGGATATGCTTTGAGTACATGTTCAATG
CCTGTAAGAGTCATGAAATTCTGCGGATGCACACACTTAATTGCTACAAATGCTGCTGGT
GGTTTAAACAGTTCATATAATGTTGGTGACATCATGATTGTTAAAGATCACATAAATATT
ATGGGATTTGCAGGAAATTCACCTTTGCAAGGACCTAATGATCATAGATTTGGACCACGA
TTTCCACCGATGAATAAAGCTTACGATCCTGAATTAATTGACATCGCTTTGAGACTTTCG
AAAGAGATGAAAATGGAAAGTGAAACAAGATGCGGTGTTTACACATGTGTGGGTGGACCT
AATTATGAGACAGTTGCTGAATTAAGAATGTGGAAGATTTTGGGAGTTGATG
>g9731.t2 Gene=g9731 Length=257
MSQIIINGNGNHYDDSHKIKNGNSYKNNNSSPPRTASPIFSYVNENYSYEIIDNIKNYLM
ARTKIRPKIGIICGTGLGHLAENLTEADSFPYEEIPNFPVSTVAGHAGRMVFGYLNGIEV
MCMQGRFHYYEGYALSTCSMPVRVMKFCGCTHLIATNAAGGLNSSYNVGDIMIVKDHINI
MGFAGNSPLQGPNDHRFGPRFPPMNKAYDPELIDIALRLSKEMKMESETRCGVYTCVGGP
NYETVAELRMWKILGVD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g9731.t2 | CDD | cd09009 | PNP-EcPNPII_like | 50 | 257 | 0 |
| 4 | g9731.t2 | Gene3D | G3DSA:3.40.50.1580 | - | 47 | 257 | 0 |
| 2 | g9731.t2 | PANTHER | PTHR11904 | METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE | 45 | 257 | 0 |
| 6 | g9731.t2 | PIRSF | PIRSF000477 | PurNPase | 42 | 257 | 0 |
| 1 | g9731.t2 | Pfam | PF01048 | Phosphorylase superfamily | 68 | 257 | 0 |
| 3 | g9731.t2 | SUPERFAMILY | SSF53167 | Purine and uridine phosphorylases | 47 | 257 | 0 |
| 5 | g9731.t2 | TIGRFAM | TIGR01697 | PNPH-PUNA-XAPA: inosine/guanosine/xanthosine phosphorylase family | 68 | 257 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004731 | purine-nucleoside phosphorylase activity | MF |
| GO:0009116 | nucleoside metabolic process | BP |
| GO:0006139 | nucleobase-containing compound metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03783 (purine-nucleoside phosphorylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
