General information for g9770.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Senecionine N-oxygenase.
Parent gene
- The parent gene g9770 is located on chr_1: 5107643..5108938 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9770 | g9770.t1 | TSS | g9770.t1 | 5107600 | 5107600 |
| chr_1 | g9770 | g9770.t1 | isoform | g9770.t1 | 5107643 | 5108938 |
| chr_1 | g9770 | g9770.t1 | exon | g9770.t1.exon1 | 5107643 | 5108938 |
| chr_1 | g9770 | g9770.t1 | cds | g9770.t1.CDS1 | 5107643 | 5108938 |
| chr_1 | g9770 | g9770.t1 | TTS | g9770.t1 | 5109102 | 5109102 |
Sequences
>g9770.t1 Gene=g9770 Length=1296
ATGAAAGTAGCAAAAGTTGCAGTGGTTGGTTGTGGTCCAAGTGGTTTGTGTGCTATTAAA
AATTGTATTTCTGAAGGATTCGAAGTTGTTGCATTTGAACAAACTTCAGAAATTGGTGGA
GAATGGAATTCAAATTTAGAAGTTGGTCCAAATGTTCACTCAAAAATATATGAAGGCTTG
GAAACAAATTTCACGAAGGAATTAATGGAATATTCAGACTTTTCTTATGATGAAAAGGTA
ACTGAATCATTTTTGACTCAAGACAAAGTTTTTAAATACTTCAAGTCATATACTAAAAAA
TTTGAACTTAAAAAATACATAAAATTTAACCATCGTGTCATTAGAATTCGACCAGTTGAT
GATCAATGGGAAATTATTGTTCAAAATCTCAAAAATGATGAATTTGAAACAATTTTTAGT
GATACAGTTTTTATATGCAACGGATTATCTGCACCTTGGATGCCAAAATTTGAAGGTCAA
AATATATTTAAAGGAAATATTCTTCATTCTCGTGATTATCGAAATACGAAGATGTTCAAA
GGTGAAAAAGTTTTGCTCATCGGTAGTGGCACAAGTGCATTTGATATGGTCATTGCTATC
GATAAAGTCGCTAAAAAAATTGTTTGGTTAAATAAAATTCAACAAACTCATGGTAAACAA
GTAAATTTAAATTTGTCATCAAATTCAATAATTAAAACATCGCAAGTCAAAAGATTTACG
AAATCTGGTGCAGAATTTGAAGATGGAAGTTTTGAAGAATTTTCAATTATTGCATTTGCA
ACAGGATATGATTTCACTTTTCCATTTTTGTCAGTTGATTCTGGAATTTCAGTTTATGAC
AAATGTGTTCAACCACTTTATAAACAATGCATCAATGCTAATCGACCATCAATGGCCATT
ATTGGTTTGCCTTGGTTTACACTTCGAATTCCTTTATTTGAATTACAAATTAAATTTTGT
ATGCAATATTGGTCAGGTAGAAAAAATCTACCATCAAGAGAAGAAATGCTGCAAGACATT
CAAGAAGATTTTAAAACTCGAAATATTACAGAAAATCTTCATAAAGCACATTACTTGGGA
TTTGCGAAACATGGATTGTATTTTGATGATTTGGCAAAAACTTCTGGAATTGAACCATTA
AAGCAAGTTCTCGTTGATTTATCGACTCATCAAGTACAACATGCATTTGAAAATTACAAA
ACATTTCGATTTTACAATTACAAAGTTTTGAATGATTTTGAATTTTCATGCAATTGTAAA
TTAAATTTGATTAAAAATGAAGATGATAAAGTATAA
>g9770.t1 Gene=g9770 Length=431
MKVAKVAVVGCGPSGLCAIKNCISEGFEVVAFEQTSEIGGEWNSNLEVGPNVHSKIYEGL
ETNFTKELMEYSDFSYDEKVTESFLTQDKVFKYFKSYTKKFELKKYIKFNHRVIRIRPVD
DQWEIIVQNLKNDEFETIFSDTVFICNGLSAPWMPKFEGQNIFKGNILHSRDYRNTKMFK
GEKVLLIGSGTSAFDMVIAIDKVAKKIVWLNKIQQTHGKQVNLNLSSNSIIKTSQVKRFT
KSGAEFEDGSFEEFSIIAFATGYDFTFPFLSVDSGISVYDKCVQPLYKQCINANRPSMAI
IGLPWFTLRIPLFELQIKFCMQYWSGRKNLPSREEMLQDIQEDFKTRNITENLHKAHYLG
FAKHGLYFDDLAKTSGIEPLKQVLVDLSTHQVQHAFENYKTFRFYNYKVLNDFEFSCNCK
LNLIKNEDDKV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g9770.t1 | Gene3D | G3DSA:3.50.50.60 | - | 5 | 403 | 0 |
| 15 | g9770.t1 | Gene3D | G3DSA:3.50.50.60 | - | 156 | 260 | 0 |
| 3 | g9770.t1 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 5 | 390 | 0 |
| 16 | g9770.t1 | PIRSF | PIRSF000332 | FMO | 2 | 221 | 0 |
| 17 | g9770.t1 | PIRSF | PIRSF000332 | FMO | 221 | 399 | 0 |
| 10 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 4 | 20 | 0 |
| 4 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 28 | 52 | 0 |
| 5 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 82 | 97 | 0 |
| 7 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 122 | 138 | 0 |
| 8 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 168 | 182 | 0 |
| 9 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 195 | 210 | 0 |
| 6 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 247 | 274 | 0 |
| 11 | g9770.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 311 | 324 | 0 |
| 1 | g9770.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 5 | 208 | 0 |
| 2 | g9770.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 232 | 379 | 0 |
| 12 | g9770.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 3 | 210 | 0 |
| 13 | g9770.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 240 | 412 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00485 (dimethylaniline monooxygenase (N-oxide forming))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed