General information for g982.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lysosomal acid lipase/cholesteryl ester hydrolase.
Parent gene
- The parent gene g982 is located on chr_3: 7250715..7251787 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g982 | g982.t1 | isoform | g982.t1 | 7248525 | 7251163 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon1 | 7248525 | 7248816 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS1 | 7248525 | 7248816 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon2 | 7248987 | 7249229 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS2 | 7248987 | 7249229 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon3 | 7249322 | 7249419 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS3 | 7249322 | 7249419 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon4 | 7249560 | 7249670 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS4 | 7249560 | 7249670 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon5 | 7249732 | 7249780 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS5 | 7249732 | 7249780 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon6 | 7250117 | 7250218 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS6 | 7250117 | 7250218 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon7 | 7250274 | 7250325 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS7 | 7250274 | 7250325 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon8 | 7250490 | 7250635 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS8 | 7250490 | 7250635 |
| chr_3 | g982 | g982.t1 | exon | g982.t1.exon9 | 7250703 | 7251163 |
| chr_3 | g982 | g982.t1 | cds | g982.t1.CDS9 | 7250703 | 7251163 |
| chr_3 | g982 | g982.t1 | TSS | g982.t1 | 7251478 | 7251478 |
| chr_3 | g982 | g982.t1 | TTS | g982.t1 | NA | NA |
Sequences
>g982.t1 Gene=g982 Length=1554
ATGAAGACAATTATCGGTGTTTGTTTACTGCTTTGTCTACTCGTGACTGTGAAGAGTGAA
GGTGGAACTGATGTTGTTATGAGCATACTCTCCCAATTTTTATCACAATCTTCACCAAAT
GTGCGAGCTACCACACCAAGCGGAATTCTTCCAAAAGTTCTAAATCCACTCAATTGGATA
CCAAAAATACCTGAAGTTCCTTGGAATCCTGATAGCGAATTAACAACACCAGAAATTATT
GTTAGACATGGATATCAAGCTGAAACCCACACAGTACAAACTGAAGATGGTTATTTGTTA
AATATGCATAGAATTCCTTGCGGAAGAGCTGGTTGTAGTGATGAAATTCGGCAACCAGTG
TTTTTACAACATGGAATTCTCGCAAGCAGTGCAGACTGGATTTTGAGTGGACCAGAAAAA
GGAATTGGATTTATACTTGCTGATCTTGGCTATGATGTTTGGCTTTCAAATTTGAGAGGA
AACACATATTCTCGTCATCATACTCAAATGAGTAACACTGATAACGAATTTTGGGATTTT
AGTTTCCATGAAATGGCAATTTTTGATATTCCTGCTGAAATTGATTACATTTATGAACAT
CGAAAACGAATAATGGGAGAAGGAGTTTTAAGCAATGACTTGATTTACGTAGGTCATAGT
ATGGGAACATCAGCATTGTTTGTTATGCTAAGTGAACTGCCTGAATATAATGAAAGAATT
AAATTATTTATTGCCTTAGCACCAGTTGCATTTATGAGCCACGTAAAATCACCACTTCGC
TTCCTCGCTCCATTCTCAAAAGATATTGAGCTCGATTTTCTCGTTAAAAAACTTAAAATT
CCTGAAAATTGGCATTCATCATTAAGAAGAGTAGATGGAATGACAAACGAATTTTTAAAA
AAGTTTTCATTAAAATTCTTGAAAATTATGAAATTCTTTGGTCAAAATGAATTCCTGCCA
CAAAATAAAATCATCAAATGGCTCTCAAAATATGGATGTGAAATGAATGAAGTTGAAAAT
CCTGCTGGTACATCAACAAAAACAGTTGTGCATTACGCACAAGAAATACAAGAGAGTGGA
AATTTCCAAAACTTCGATTACGGCAAAGAAGAAAATGAACGTCGTTATGGTTCAGAGCAT
CCCACACAGTACAATTTATCAAATGTCAATGTTCCAATTGCACTTTTCTATGCACAAAAT
GATTGGCTTGCTGGCTATCAAGATGTTAAGAAATTATATACAAATTTACATTCTGCAATT
ATAATAAAAGAATATTCATTAAAAATGTCAGAGAAACCACCAAAGAAAACTTTATTGCAA
AAGTATGAAATTCCAGTTGAATTTCTTGATTTTGATTATTTAAAAACATGCACAGAAGCA
AAAACTGTTGAAAAAATTGTGAAAATATTGCGTTCAGGTGAAGAAGGTTATTATCCAGAT
CTTCAGAAATTTGCAGAAGATAAGCTTAAAGAATTGAAGCCAAATAGCAAAGTTTTTAGA
GTTGAAGAACCAGCAGTGAAAATTCAAACACCGATGAGGAAAAAGCGCAATTGA
>g982.t1 Gene=g982 Length=517
MKTIIGVCLLLCLLVTVKSEGGTDVVMSILSQFLSQSSPNVRATTPSGILPKVLNPLNWI
PKIPEVPWNPDSELTTPEIIVRHGYQAETHTVQTEDGYLLNMHRIPCGRAGCSDEIRQPV
FLQHGILASSADWILSGPEKGIGFILADLGYDVWLSNLRGNTYSRHHTQMSNTDNEFWDF
SFHEMAIFDIPAEIDYIYEHRKRIMGEGVLSNDLIYVGHSMGTSALFVMLSELPEYNERI
KLFIALAPVAFMSHVKSPLRFLAPFSKDIELDFLVKKLKIPENWHSSLRRVDGMTNEFLK
KFSLKFLKIMKFFGQNEFLPQNKIIKWLSKYGCEMNEVENPAGTSTKTVVHYAQEIQESG
NFQNFDYGKEENERRYGSEHPTQYNLSNVNVPIALFYAQNDWLAGYQDVKKLYTNLHSAI
IIKEYSLKMSEKPPKKTLLQKYEIPVEFLDFDYLKTCTEAKTVEKIVKILRSGEEGYYPD
LQKFAEDKLKELKPNSKVFRVEEPAVKIQTPMRKKRN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g982.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 65 | 432 | 4.5E-112 |
| 2 | g982.t1 | PANTHER | PTHR11005:SF88 | LIPASE | 68 | 427 | 2.1E-115 |
| 3 | g982.t1 | PANTHER | PTHR11005 | LYSOSOMAL ACID LIPASE-RELATED | 68 | 427 | 2.1E-115 |
| 1 | g982.t1 | Pfam | PF04083 | Partial alpha/beta-hydrolase lipase region | 77 | 136 | 1.6E-23 |
| 9 | g982.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 10 | g982.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 11 | g982.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 12 | g982.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 8 | g982.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 517 | - |
| 4 | g982.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 66 | 420 | 2.99E-47 |
| 6 | g982.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 5 | g982.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006629 | lipid metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01052 (lysosomal acid lipase/cholesteryl ester hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed