Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cullin-5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g9899 g9899.t9 isoform g9899.t9 5913488 5914093
chr_1 g9899 g9899.t9 exon g9899.t9.exon1 5913488 5913885
chr_1 g9899 g9899.t9 cds g9899.t9.CDS1 5913847 5913885
chr_1 g9899 g9899.t9 exon g9899.t9.exon2 5913956 5914093
chr_1 g9899 g9899.t9 cds g9899.t9.CDS2 5913956 5914093
chr_1 g9899 g9899.t9 TTS g9899.t9 5914301 5914301
chr_1 g9899 g9899.t9 TSS g9899.t9 NA NA

Sequences

>g9899.t9 Gene=g9899 Length=536
TATTTGCGTGGAATCAACGTCCTAATGATAAAGTTTCATATGAGAATTTAAGGCTCGCAA
CTGAATTACCTGACCCTGAGCTAAGAAGAACTCTTTGGTCGCTTGTGGCGTTTCCTAAAC
TGAAAAGACAGCTTTTAAATTATGATCCAATCGTTCAAGTGCCAAAAGATTTTGACGAGA
ACACGGTTTTTTGGATAAATCAAGAATTCTCAATTATTAAAAATGGAAAGCCTCAAAAAA
GAGGTAAAATTAATTTAATTGGGAGACTTCAACTCAGTACAGAGCGCTCTCAGCAAGAAG
ACAATCAATCGATTGTGCAATTGAGAATTCTCAGAACACAAGAAGCAATCATTAAAATTA
TGAAAATGAGAAAGAGAATGTCGAATGCTGCTTTACAGTCTGAACTTATTGAGATATTGA
AAAATATGTTTTTACCCTCGAAAAAAATGATTAAAGAGCAATTGGAGTGGTTAATTGAAC
AAAAATATCTTGCTCGTGACGACAGTGATATAAACGTATTTAATTATATGGCTTGA

>g9899.t9 Gene=g9899 Length=58
MKMRKRMSNAALQSELIEILKNMFLPSKKMIKEQLEWLIEQKYLARDDSDINVFNYMA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g9899.t9 Gene3D G3DSA:1.10.10.10 winged helix repressor DNA binding domain 1 58 1.2E-22
2 g9899.t9 PANTHER PTHR11932 CULLIN 1 58 4.5E-26
3 g9899.t9 PANTHER PTHR11932:SF76 CULLIN-5 1 58 4.5E-26
1 g9899.t9 Pfam PF10557 Cullin protein neddylation domain 1 50 5.2E-15
6 g9899.t9 ProSitePatterns PS01256 Cullin family signature. 31 58 -
5 g9899.t9 SMART SM00884 Cullin_Nedd8_2 1 52 1.1E-10
4 g9899.t9 SUPERFAMILY SSF46785 Winged helix DNA-binding domain 1 58 1.13E-16

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006511 ubiquitin-dependent protein catabolic process BP
GO:0031625 ubiquitin protein ligase binding MF
GO:0031461 cullin-RING ubiquitin ligase complex CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values