Gene loci information

Transcript annotation

  • This transcript has been annotated as Bifunctional 3’-phosphoadenosine 5’-phosphosulfate synthase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11605 g11605.t4 TTS g11605.t4 17424500 17424500
chr_1 g11605 g11605.t4 isoform g11605.t4 17424763 17427124
chr_1 g11605 g11605.t4 exon g11605.t4.exon1 17424763 17425817
chr_1 g11605 g11605.t4 cds g11605.t4.CDS1 17424763 17425817
chr_1 g11605 g11605.t4 exon g11605.t4.exon2 17425896 17426037
chr_1 g11605 g11605.t4 cds g11605.t4.CDS2 17425896 17426037
chr_1 g11605 g11605.t4 exon g11605.t4.exon3 17426115 17426633
chr_1 g11605 g11605.t4 cds g11605.t4.CDS3 17426115 17426633
chr_1 g11605 g11605.t4 exon g11605.t4.exon4 17426726 17426848
chr_1 g11605 g11605.t4 cds g11605.t4.CDS4 17426726 17426848
chr_1 g11605 g11605.t4 exon g11605.t4.exon5 17426971 17427124
chr_1 g11605 g11605.t4 cds g11605.t4.CDS5 17426971 17427003
chr_1 g11605 g11605.t4 TSS g11605.t4 17427117 17427117

Sequences

>g11605.t4 Gene=g11605 Length=1993
AGTAATCAGTTGAATTTTGTTCATAGAGGAAATCAAACGTATATTTTTCTCGTCACATAT
TCTCTCACTCTCCAAAAAAAGTGTATATAATTTTGAAGTTCTCTTTAAAAAAATTTTAAA
AATGAGAAGGAGAATAGCAAATGACGATAACAATTGTCTTCAAGTGGCAACAAATGTAAC
TGAACAAAAGCATCATGTTACTCGAGAGACACGCGGACAAAATTTGGGATTATGCCGTGG
CTTTCGAGGTTGTACAGTTTGGTTGACGGGATTATCGGGTGCAGGTAAAACTTCTATTGG
TTTTGAACTTGAAGCACTTCTAGTATCAAAGGGCATTCCTTCATATGGACTCGATGGTGA
CAACATTCGTACGGGATTAAATAAAAACTTAGGCTTCTCGCAAGCAGATCGCGAAGAAAA
TATTCGTCGAGTGGCTGAAGTGGCTAAATTGTTTGCCGATAGTGGTGTTGTGGCAATTTG
TAGTTTTGTTTCTCCATTTGCTGAAGATCGTGATATGGCGCGAAGAATTCATAAAGAGGC
CGATTTGAAGTTTTATGAAATATTCGTCGATACACCATTAAACGTTTGCGAGTCTCGTGA
CGTCAAAGGTCTTTACAAAAAAGCTCGAGAAGGTGCAATTCAAGGCTTTACTGGTGTAAC
GCAAGCTTATGAAGCTCCAGATAAACCGGATTTAATTGTAAAAACTGAAGGTCTTTCCAT
TCAAGAATCAACTCAATGCGTTATTGAGCTGCTAGTTAAAGAAAATATCATCCCAAAGAG
TATCACAGATGTTTATGGAATCCATGAATTATTTGTTCCATCACACCGTCGTTCCGCAAT
GCTTGCAGAAGCTCAAACTTTGCCCACATTAGAGATCAGTGAAGTTGAATGTCAATGGCT
TCAAGTACTAGCGGAGGGTTGGGCTTCACCTCTTAAAGGTTTTATGCGTGAAAAGGACTA
TCTTCAAACATTACATTTTAATTGTATTGTGACAGATGACGGTACCGTCATTAATCATTC
TGTCCCGATTGTTTTATCGCTATCAGAGAGTGATAAGAGTCGATTACAAGATGTTAGCGC
ATTCACATTAACTTATGATAAGAAACCGATCGCAATTTTACGTAACCCTGAATTCTTTTA
TCAACGAAAAGAGGAACGTTGTGCTCGACAATTTGGCACAGCTAATCCCAATCATCCTTA
TATCAAGATGATTTTAGAGTCTGGTGATTTTCTTGTTGGTGGTGATTTGGAAGTCTTAGA
TAGAATTAAATGGAATGATGGTTTAGATCAATATCGTTTAACACCAAATGAAATTCGCAA
CAAGATTAAGGAAATGAATGCAGATGCAGTTTTTGCTTTTCAATTGAGAAATCCTATTCA
TAACGGTCATGCGTTATTAATGAAAGATTGTAAGCGACAATTATTAGATCGGGGTTATAA
AAAACCAGTATTACTTTTACATCCACTCGGCGGATGGACGAAAGATGACGATGTTCCTTT
GCCAACCAGAATGGCTCAACACCAAGCTGTCATGGACGCAGGAGTGTTGGATCGTGATAG
CACAATTCTTGCCATTTTTCCTTCGCCAATGATGTATGCGGGACCAACTGAAGTTCAATG
GCATGCTAAATCTCGAATGAATGCCGGTGCAAGCTTTTATATTGTAGGTCGCGATCCAGC
CGGAATGCCACATCCCAATAAAGAAATGTACCCTGATGGTAATCTTTATGATGACACACA
TGGCAGCAGAGTTTTGAAAATGGCTCCGGGACTTGATAATATCGTTATTTTGCCATTTAG
AGTGGCAGCTTATGATACATCTGTTAACGAAATGGCATTTTTCGACCCTAAACGTAAAGA
AGACTTTTTATTCATATCTGGCACAAAAATGCGTGGATTTGCAAAAAGTGGTGAGTTGCC
GCCAAAAGGATTTATGGAACCCAAAGCATGGAGTATTCTTGCTGAATATTATCAATCACT
TAATAAGAACTAG

>g11605.t4 Gene=g11605 Length=623
MRRRIANDDNNCLQVATNVTEQKHHVTRETRGQNLGLCRGFRGCTVWLTGLSGAGKTSIG
FELEALLVSKGIPSYGLDGDNIRTGLNKNLGFSQADREENIRRVAEVAKLFADSGVVAIC
SFVSPFAEDRDMARRIHKEADLKFYEIFVDTPLNVCESRDVKGLYKKAREGAIQGFTGVT
QAYEAPDKPDLIVKTEGLSIQESTQCVIELLVKENIIPKSITDVYGIHELFVPSHRRSAM
LAEAQTLPTLEISEVECQWLQVLAEGWASPLKGFMREKDYLQTLHFNCIVTDDGTVINHS
VPIVLSLSESDKSRLQDVSAFTLTYDKKPIAILRNPEFFYQRKEERCARQFGTANPNHPY
IKMILESGDFLVGGDLEVLDRIKWNDGLDQYRLTPNEIRNKIKEMNADAVFAFQLRNPIH
NGHALLMKDCKRQLLDRGYKKPVLLLHPLGGWTKDDDVPLPTRMAQHQAVMDAGVLDRDS
TILAIFPSPMMYAGPTEVQWHAKSRMNAGASFYIVGRDPAGMPHPNKEMYPDGNLYDDTH
GSRVLKMAPGLDNIVILPFRVAAYDTSVNEMAFFDPKRKEDFLFISGTKMRGFAKSGELP
PKGFMEPKAWSILAEYYQSLNKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g11605.t4 CDD cd02027 APSK 45 195 0.0000
15 g11605.t4 CDD cd00517 ATPS 247 616 0.0000
10 g11605.t4 Gene3D G3DSA:3.40.50.300 - 15 221 0.0000
12 g11605.t4 Gene3D G3DSA:3.10.400.10 Sulfate adenylyltransferase 226 385 0.0000
11 g11605.t4 Gene3D G3DSA:3.40.50.620 HUPs 386 623 0.0000
6 g11605.t4 Hamap MF_00065 Adenylyl-sulfate kinase [cysC]. 41 215 36.0827
4 g11605.t4 PANTHER PTHR11055 BIFUNCTIONAL 3’-PHOSPHOADENOSINE 5’-PHOSPHOSULFATE SYNTHASE 8 623 0.0000
5 g11605.t4 PANTHER PTHR11055:SF16 BIFUNCTIONAL 3’-PHOSPHOADENOSINE 5’-PHOSPHOSULFATE SYNTHASE 2 8 623 0.0000
3 g11605.t4 Pfam PF01583 Adenylylsulphate kinase 42 197 0.0000
2 g11605.t4 Pfam PF14306 PUA-like domain 227 380 0.0000
1 g11605.t4 Pfam PF01747 ATP-sulfurylase 389 616 0.0000
8 g11605.t4 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 41 213 0.0000
9 g11605.t4 SUPERFAMILY SSF88697 PUA domain-like 227 384 0.0000
7 g11605.t4 SUPERFAMILY SSF52374 Nucleotidylyl transferase 390 617 0.0000
13 g11605.t4 TIGRFAM TIGR00455 apsK: adenylyl-sulfate kinase 38 211 0.0000
14 g11605.t4 TIGRFAM TIGR00339 sopT: sulfate adenylyltransferase 230 615 0.0000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0000103 sulfate assimilation BP
GO:0005524 ATP binding MF
GO:0004020 adenylylsulfate kinase activity MF
GO:0004781 sulfate adenylyltransferase (ATP) activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values