Gene loci information

Transcript annotation

  • This transcript has been annotated as Mediator of RNA polymerase II transcription subunit 27.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1180 g1180.t1 TSS g1180.t1 8507199 8507199
chr_3 g1180 g1180.t1 isoform g1180.t1 8507262 8508282
chr_3 g1180 g1180.t1 exon g1180.t1.exon1 8507262 8507333
chr_3 g1180 g1180.t1 cds g1180.t1.CDS1 8507262 8507333
chr_3 g1180 g1180.t1 exon g1180.t1.exon2 8507391 8507564
chr_3 g1180 g1180.t1 cds g1180.t1.CDS2 8507391 8507564
chr_3 g1180 g1180.t1 exon g1180.t1.exon3 8507621 8507872
chr_3 g1180 g1180.t1 cds g1180.t1.CDS3 8507621 8507872
chr_3 g1180 g1180.t1 exon g1180.t1.exon4 8507929 8508282
chr_3 g1180 g1180.t1 cds g1180.t1.CDS4 8507929 8508282
chr_3 g1180 g1180.t1 TTS g1180.t1 8508348 8508348

Sequences

>g1180.t1 Gene=g1180 Length=852
ATGGAAAATCAAATAAATACAGCTTTAAATGCAATTAAAAATCTTCGTTCAAGTGTTTCT
AATATATTTGAGACAACAATATCGTCAGATATTTCTCAGTTGATTGAAATTCAAGACTCA
ATTGCTGCTGTGAGTGCAAATTTTAAAGATGTCGAAGGATCTATAAATAATTTAACAATA
CCAGCAGTTCCTTCATTGGGAAACACTGCTTTTCTCAACTATGAAACAGACTTAGAGAAA
CAAAATATGTATAGTGAACTTGTTAATGGATACAAATGGTATGAAAAGACTCATCAATAC
TCATCTATGGCAAGCACAATGTTACAACACAATTCTCTTAAAAGATCATATTACTCAACA
TCGAATAAAAGACGAAGATTATTGACAGGAGCACACACTGTGCCTCCAAAGTTGGTCGAT
CAGATTATCAGTACATTCAATTTGAATAATATGCAACTTAAAATTTATCGACCTTTTGCT
ACAAATGCATTTCTACATATTAATCTAAGCAGAGTATTGAAAGCAGCAATAATTCTTCGA
GGTCTCATGATTGAATGGATTACAGTTAAAGGATTTAATGAGTCACTTGATAACGTAGAT
GACCATTGGACTGAATCGAGATATGCTGTCTTCCGAAAAGTTCAAAAAGCTGCTCATTCA
GCTATGCTTCATTTCTACTCACCGACATTTGCCGAATTGGCAATAAGAAGTTTTATAACA
TGGTTCGGAAGCTACTCGACTCTGTTCAGTGAACCTTGCAAAAAATGTGGAAAACATTTA
TTAAACGGATTACCACCAACATTTCGAGATGTTCGCTCGTTAGATCCGATTCATGAAGAG
TGTAGAAATTAA

>g1180.t1 Gene=g1180 Length=283
MENQINTALNAIKNLRSSVSNIFETTISSDISQLIEIQDSIAAVSANFKDVEGSINNLTI
PAVPSLGNTAFLNYETDLEKQNMYSELVNGYKWYEKTHQYSSMASTMLQHNSLKRSYYST
SNKRRRLLTGAHTVPPKLVDQIISTFNLNNMQLKIYRPFATNAFLHINLSRVLKAAIILR
GLMIEWITVKGFNESLDNVDDHWTESRYAVFRKVQKAAHSAMLHFYSPTFAELAIRSFIT
WFGSYSTLFSEPCKKCGKHLLNGLPPTFRDVRSLDPIHEECRN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
3 g1180.t1 PANTHER PTHR13130:SF4 MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 27 4 147 0
5 g1180.t1 PANTHER PTHR13130 34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED 4 147 0
2 g1180.t1 PANTHER PTHR13130:SF4 MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 27 159 282 0
4 g1180.t1 PANTHER PTHR13130 34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED 159 282 0
1 g1180.t1 Pfam PF11571 Mediator complex subunit 27 201 282 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0016592 mediator complex CC

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values