General information for g13949.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g13949 is located on chr_1: 33556394..33558250 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13949 | g13949.t1 | TSS | g13949.t1 | 33556342 | 33556342 |
| chr_1 | g13949 | g13949.t1 | isoform | g13949.t1 | 33556394 | 33558250 |
| chr_1 | g13949 | g13949.t1 | exon | g13949.t1.exon1 | 33556394 | 33557191 |
| chr_1 | g13949 | g13949.t1 | cds | g13949.t1.CDS1 | 33556394 | 33557191 |
| chr_1 | g13949 | g13949.t1 | exon | g13949.t1.exon2 | 33557259 | 33557440 |
| chr_1 | g13949 | g13949.t1 | cds | g13949.t1.CDS2 | 33557259 | 33557440 |
| chr_1 | g13949 | g13949.t1 | exon | g13949.t1.exon3 | 33557502 | 33557629 |
| chr_1 | g13949 | g13949.t1 | cds | g13949.t1.CDS3 | 33557502 | 33557629 |
| chr_1 | g13949 | g13949.t1 | exon | g13949.t1.exon4 | 33557688 | 33557796 |
| chr_1 | g13949 | g13949.t1 | cds | g13949.t1.CDS4 | 33557688 | 33557796 |
| chr_1 | g13949 | g13949.t1 | exon | g13949.t1.exon5 | 33557857 | 33558250 |
| chr_1 | g13949 | g13949.t1 | cds | g13949.t1.CDS5 | 33557857 | 33558250 |
| chr_1 | g13949 | g13949.t1 | TTS | g13949.t1 | 33558294 | 33558294 |
Sequences
>g13949.t1 Gene=g13949 Length=1611
ATGAGAATCATAAGTCATTCGGATACAATTGTATCGAGAATTAAAAGTGAAATTTTTGAA
ATAAACCGTAATGAATCAGAATGGACAAAGAAAGTTCCATCAATTCCTGAATTCTACTCA
AATCGTGAATTATTTGTCACTGGTGCAACTGGATTTATTGGTAAAGTTTTGATAGAAAAA
TTGTTAAGGTCATGCAGTGACCTTAAATGCATTTACATATTATTGAGGCCAAAGAAAGGC
AAGTCTGTAGATGAACGATTGAAGCAATTGATTGAGTTGCCATTGTTTGAACCAATCAAG
AATTTCAAGCCTCAACTTTTCGACAAGCTGATACCGATTACAGGTGATGTCACTGAAATT
AATTTGGGACTTTCTGATTCTGACATGGTCCGAATGAAGAATGTTTCTGTCATTTTTCAT
TCTGCCGCGAGTGTTCGATTTGACGACACATTGCGCTATGCTGTATTCATGAATACACGT
GGCACAAGAGAGATTATGAAATTTGCAAAGAATTTGAAGAACATCAAAGTTCTTATGCAC
GTGTCAACGACCTATTCTCATCCTGAATTGAAGACTGTCAATGAGGTCATACATGAGCCA
TATGTTGATTGGAAGAAAACAATTGAAATTTGTGAACGATTTGATGACAATATTTTGAGC
ATTTTAACTCAACAATACACAGGTTTTATGCCGAACACCTACACATTTACAAAAGCATTA
GCAGAGCAGGTTGTTGCAGATGAGAAGGATAATTTGCCGTTAGCACTATTTAGGCCATCC
ATTGTTATTGCATCAATGACAGAGCCATTTCCTGGGTGGATCGACAATTTTAATGGTCCA
ATTGGATTATTAGTAGGTTCAGGTTTAGGCATCACTCGGACAATGTACTGTGACCCTGAT
AATATTGCTGATTTCACACCTGTTGATGTTTGTGTTAAAGCAATGATTATTTCAGCATGG
AAACGAGCTCATGAACCCAAAAATTCATTGCCGATAATTAATTGTTGCTTGTCAAATTAT
CGAAATGCAACATTAGAACAAATTATTGATATGGGAAAATCATTGATGAATGATATTCCA
ATACAAAAAATGCTTTGGGCTCCTGGTGGTGGACTTACAAGATGTCGATTCAAGAATTTT
CTCAAAGTAATTTTTGTTCAACTTCTTCCAGCTTTGATTATTGATCAAATTCTTAAAGCT
TTTGGTCATAAACCTTTCTTAGCAAAACTTCAAAGAAAAATTTATGAAGCAAACTTGGCT
CTTGACTATTTTATTCTAAACAATTGGCATTTTAAGAATGACAATTTTATGGCTCTTTGT
TTGCATCTCAAATTGCAAGATTTGAAAGCTTTTGACTTTCACAGTTTTCTTACATATGAT
CTTGTTTTGTACTTTAGAAATGCATCACTCGGTGCACGCAAGTTTTTACTTGGAGAAGAA
GAAGACAATATACTGCAGGCACAAACAAAATTGAAAAGACTAAAACTTTTGGATGCAATT
ATAAAAACCTTCATTTATTGCATTCTGTTTTGGTATTTTTTGATTAAATACGATACATTT
GGATTGAGTAGAATTTTTTGTGGATTAGTTAAGAGATATCATGATTATTAA
>g13949.t1 Gene=g13949 Length=536
MRIISHSDTIVSRIKSEIFEINRNESEWTKKVPSIPEFYSNRELFVTGATGFIGKVLIEK
LLRSCSDLKCIYILLRPKKGKSVDERLKQLIELPLFEPIKNFKPQLFDKLIPITGDVTEI
NLGLSDSDMVRMKNVSVIFHSAASVRFDDTLRYAVFMNTRGTREIMKFAKNLKNIKVLMH
VSTTYSHPELKTVNEVIHEPYVDWKKTIEICERFDDNILSILTQQYTGFMPNTYTFTKAL
AEQVVADEKDNLPLALFRPSIVIASMTEPFPGWIDNFNGPIGLLVGSGLGITRTMYCDPD
NIADFTPVDVCVKAMIISAWKRAHEPKNSLPIINCCLSNYRNATLEQIIDMGKSLMNDIP
IQKMLWAPGGGLTRCRFKNFLKVIFVQLLPALIIDQILKAFGHKPFLAKLQRKIYEANLA
LDYFILNNWHFKNDNFMALCLHLKLQDLKAFDFHSFLTYDLVLYFRNASLGARKFLLGEE
EDNILQAQTKLKRLKLLDAIIKTFIYCILFWYFLIKYDTFGLSRIFCGLVKRYHDY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g13949.t1 | CDD | cd05236 | FAR-N_SDR_e | 42 | 360 | 2.44108E-130 |
| 14 | g13949.t1 | CDD | cd09071 | FAR_C | 385 | 477 | 5.1634E-22 |
| 7 | g13949.t1 | Coils | Coil | Coil | 477 | 497 | - |
| 6 | g13949.t1 | Gene3D | G3DSA:3.40.50.720 | - | 21 | 367 | 1.4E-71 |
| 3 | g13949.t1 | PANTHER | PTHR11011:SF24 | FATTY ACYL-COA REDUCTASE | 32 | 515 | 3.5E-200 |
| 4 | g13949.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 32 | 515 | 3.5E-200 |
| 1 | g13949.t1 | Pfam | PF07993 | Male sterility protein | 46 | 315 | 2.9E-72 |
| 2 | g13949.t1 | Pfam | PF03015 | Male sterility protein | 387 | 478 | 2.6E-19 |
| 10 | g13949.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 379 | - |
| 12 | g13949.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 380 | 402 | - |
| 8 | g13949.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 403 | 495 | - |
| 11 | g13949.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 496 | 514 | - |
| 9 | g13949.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 515 | 536 | - |
| 5 | g13949.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 35 | 327 | 1.45E-32 |
| 15 | g13949.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 496 | 515 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13356 (alcohol-forming fatty acyl-CoA reductase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed