General information for g14219.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alanine aminotransferase 1.
Parent gene
- The parent gene g14219 is located on chr_1: 35491970..35501657 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14219 | g14219.t5 | isoform | g14219.t5 | 35490780 | 35495089 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon1 | 35490780 | 35491152 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon2 | 35491812 | 35492043 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS1 | 35491970 | 35492043 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon3 | 35492103 | 35492151 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS2 | 35492103 | 35492151 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon4 | 35492609 | 35492668 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS3 | 35492609 | 35492668 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon5 | 35492737 | 35492766 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS4 | 35492737 | 35492766 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon6 | 35493918 | 35494047 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS5 | 35493918 | 35494047 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon7 | 35494107 | 35494448 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS6 | 35494107 | 35494448 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon8 | 35494515 | 35494727 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS7 | 35494515 | 35494727 |
| chr_1 | g14219 | g14219.t5 | exon | g14219.t5.exon9 | 35494791 | 35495089 |
| chr_1 | g14219 | g14219.t5 | cds | g14219.t5.CDS8 | 35494791 | 35495089 |
| chr_1 | g14219 | g14219.t5 | TSS | g14219.t5 | NA | NA |
| chr_1 | g14219 | g14219.t5 | TTS | g14219.t5 | NA | NA |
Sequences
>g14219.t5 Gene=g14219 Length=1728
AAGGTGTGATTTCGTTCATAATTTACTTTTAAGGAGTAAATGTTTCAATTTCATTTTTGT
TTCAATATTATTTTGATTTCCATTTTCATATCAATTCTTTTTGTGTGTAAAAAATTCATT
GTAGTCTCGTCTCGTCTCTTTTCTAGACTCTCTCGCGTAAAAAGAAAAGTAAATATATTT
TCGGAATCAAAATAAAATCAAGTGTATTTGGCCAGTGTGTGTAGGAAAATATATATCTAG
ATTTTTCACCAGTTGATTATTTTCATTCGGTGCTAATAGATCTCGACGAAAAAATTCTTT
AGAATTACTGGATCATAATTGAATTGAAAAATATTGAATAATCTTGATAGCAATCGTTCT
CATTAAAAGAAAAAGAGCTGTTTAACTATGGCAACCCTCACTTCATTCATTTCTCATTCA
CTTCGTTCAACAAAACATTTTAGTCGTATTATACACCGGTCGTCGTATAAGTCCGTTTTA
AGCTATCATCTAAATAATTCTGAAAATTTTTGGTCATCATCACCAAGAGAAATGTCGACT
TTAGGAAAATGTCTTTCTATTGATAACATCAATCCATGCATAAAAACTATGGAATATGCT
GTGAGAGGACCTCTCGTAATTCGTGCGGCAGCTATTGAAAAAGAATTGGAGCAGGGCGTT
AAGAAGCCATTTAATGAAGTCATACGAGCAAATATTGGCGATTGCCATGCTATGGGTCAA
ATTCCCATTACTTTTATTCGTCAAGTACTTGGTCTGTGCGTTTATGAGAAACTTTTTGAT
GATCCAACAATTCCCTCGGATGCTAAACAACGTGCACGCGACATCTTGAATGGTTGCAAA
GGTGGCTCATTGGGCTCTTATTCAGACTCGACTGGTATCGAAGTCATAAGAAGACATTGT
GCTGAGTACATTCATAAACGTGATGGAATCCCATCGAATGCTGACGACATTATTTTGTCA
GCTGGTGCTTCTGGCAGCATTAAAGCAATTCTTGCACTTCTTCGCTGTGATATTGATGGA
AAAAGACCAGGTGTAATGGTACCTATTCCACAATATCCATTATATTCTGCTACAATCGCA
GAATTTGACATGCAACAAATTGGATATTACTTAGATGAATCAAACAATTGGGGATTAGAT
ATTGCTGAATTGCAAAGATCGATTGATGAGGCAAAGAAAAATTCATATCCACGTGCAATT
GTTATTATTAATCCTGGTAATCCAACTGGACAAGTTTTAACGAAAGAAAATATTGAAGCA
GTAATCAAATTTGCTCACAAAGAAAATCTCTTTATTTTTGCTGATGAAGTATATCAAGAT
AATATCTATGCACCTGATTCAAAATTTTTTTCATTCAAAAAAGTTTTAACTGAAATGGGA
GAGCCATATTCAAAAATGGCTATGGGAAGTTTTATGAGTTGCTCCAAAGGCTATATGGGT
GAATGTGGAATTAGAGGTGGCTATTGTGAACTCATTAATGTTTGTCCTGAAGTAAGAGCA
GCTCTCAACAAAAGCATATCAGCACAATTGTGCCCTACAACCGTTGGTCAAGCTTGTATT
GATTGTGTTGTAAATCCACCTACTCCATTTGAGCCATCCTATGATCTTTTTATGAAAGAA
AAGGGAGAAGTGCTTCAGTCACTTAAAGAGCGTGCATTGTTGGTTGTTTCAACTTTTAAT
TCAATTGAGGGCTTAAGTTGTAACCCCGCACAAGGCGCAATGTATTGT
>g14219.t5 Gene=g14219 Length=399
MSTLGKCLSIDNINPCIKTMEYAVRGPLVIRAAAIEKELEQGVKKPFNEVIRANIGDCHA
MGQIPITFIRQVLGLCVYEKLFDDPTIPSDAKQRARDILNGCKGGSLGSYSDSTGIEVIR
RHCAEYIHKRDGIPSNADDIILSAGASGSIKAILALLRCDIDGKRPGVMVPIPQYPLYSA
TIAEFDMQQIGYYLDESNNWGLDIAELQRSIDEAKKNSYPRAIVIINPGNPTGQVLTKEN
IEAVIKFAHKENLFIFADEVYQDNIYAPDSKFFSFKKVLTEMGEPYSKMAMGSFMSCSKG
YMGECGIRGGYCELINVCPEVRAALNKSISAQLCPTTVGQACIDCVVNPPTPFEPSYDLF
MKEKGEVLQSLKERALLVVSTFNSIEGLSCNPAQGAMYC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g14219.t5 | CDD | cd00609 | AAT_like | 82 | 399 | 0 |
| 5 | g14219.t5 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 3 | 79 | 0 |
| 6 | g14219.t5 | Gene3D | G3DSA:3.40.640.10 | - | 108 | 356 | 0 |
| 2 | g14219.t5 | PANTHER | PTHR11751 | ALANINE AMINOTRANSFERASE | 5 | 398 | 0 |
| 3 | g14219.t5 | PANTHER | PTHR11751:SF308 | ALANINE AMINOTRANSFERASE 1 | 5 | 398 | 0 |
| 1 | g14219.t5 | Pfam | PF00155 | Aminotransferase class I and II | 71 | 399 | 0 |
| 4 | g14219.t5 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 77 | 399 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009058 | biosynthetic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
KEGG
Orthology
- This transcript has been annotated with K00814 (alanine transaminase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
