General information for g194.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase.
Parent gene
- The parent gene g194 is located on chr_3: 1641991..1642590 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g194 | g194.t4 | TSS | g194.t4 | 1640496 | 1640496 |
| chr_3 | g194 | g194.t4 | isoform | g194.t4 | 1640804 | 1641630 |
| chr_3 | g194 | g194.t4 | exon | g194.t4.exon1 | 1640804 | 1641630 |
| chr_3 | g194 | g194.t4 | cds | g194.t4.CDS1 | 1640840 | 1641628 |
| chr_3 | g194 | g194.t4 | TTS | g194.t4 | 1642202 | 1642202 |
Sequences
>g194.t4 Gene=g194 Length=827
TCTTCATATACACACTATCCGACAATAAGTACTGACATGTTAAGACGAGTGGCAAATAGA
ACGTTAACTTATAATAATCGAAGTCATGTAGCAAGAAATCCATTCCTCACATGGGCAAAA
CTTTCATATTATCGTCTCTTTGCATCATTCTATGGTATTGTTGGACGATGCGCAGAAACA
GTCATGGTAAATTCTTCATGGACTGAAAATCACATCAATTCTATATGGAATATACCACTT
AAAACGCATCGCGTTTATCCACCATGTGAAGTATCTCATTTGAAAGAGATTCAACATGTA
CCAAGTAGTGATGGAAACATTTATATTCTGTCAGTTGGACAATATCGGCCTGAAAAAGAT
CATCCTTTGATTCTCCAAAGCCTCTACGAACTGAGAACTTTGCTTGAGCGTGATGAAGAG
CTATGGGAAAGAATTCGTTTAATTTTTGTCGGCTCATGTCGTAATGATGAGGATCTCGAA
CGTGTTCAGAATTTGCGAGATTTCAGTCGACACTTGGCATTAGATGAACATGTTGAATTC
AAAGTGAATGCCTCTTATCAAGAATTGATTCAATGTTACCAAAAATCGCTAATTGGTATT
CATGCAATGTGGAATGAACATTTCGGCATAAGTGTTGTTGAACTAATGGCTGCTGGTCTT
ATCATGATTGCGAATAGATCGGGTGGACCGAGAATGGATATAATTGAAACATCAGAAGGT
AGTCAAACTGGTTACCTTGCCGATAGCGCTGAAGACTATGCTCGCTGTCTAGAAACAATT
TTATACAACAGCAGAGAGCAAAATGATAAAATTAGAAATGCTGCAAG
>g194.t4 Gene=g194 Length=263
MLRRVANRTLTYNNRSHVARNPFLTWAKLSYYRLFASFYGIVGRCAETVMVNSSWTENHI
NSIWNIPLKTHRVYPPCEVSHLKEIQHVPSSDGNIYILSVGQYRPEKDHPLILQSLYELR
TLLERDEELWERIRLIFVGSCRNDEDLERVQNLRDFSRHLALDEHVEFKVNASYQELIQC
YQKSLIGIHAMWNEHFGISVVELMAAGLIMIANRSGGPRMDIIETSEGSQTGYLADSAED
YARCLETILYNSREQNDKIRNAA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g194.t4 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 76 | 263 | 0 |
| 3 | g194.t4 | PANTHER | PTHR45919 | GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE | 1 | 263 | 0 |
| 2 | g194.t4 | Pfam | PF15924 | ALG11 mannosyltransferase N-terminus | 1 | 64 | 0 |
| 1 | g194.t4 | Pfam | PF00534 | Glycosyl transferases group 1 | 86 | 262 | 0 |
| 4 | g194.t4 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 27 | 261 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004377 | GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity | MF |
| GO:0016757 | glycosyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
