General information for g2835.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Apyrase.
Parent gene
- The parent gene g2835 is located on chr_3: 20500421..20502281 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2835 | g2835.t2 | isoform | g2835.t2 | 20499957 | 20501830 |
| chr_3 | g2835 | g2835.t2 | exon | g2835.t2.exon1 | 20499957 | 20499958 |
| chr_3 | g2835 | g2835.t2 | exon | g2835.t2.exon2 | 20500143 | 20501830 |
| chr_3 | g2835 | g2835.t2 | cds | g2835.t2.CDS1 | 20500421 | 20501365 |
| chr_3 | g2835 | g2835.t2 | TSS | g2835.t2 | 20502370 | 20502370 |
| chr_3 | g2835 | g2835.t2 | TTS | g2835.t2 | NA | NA |
Sequences
>g2835.t2 Gene=g2835 Length=1690
GGTTTGATATATATTTTAAAATAATTACTTGATATTAAATAAATATTTTTTAATAGGAAA
TCATGAATTCGATAGAGGTGTACCTGAGGTTGTGCATTTTCTTGAACATTTAATTTCGCC
TGTAGTTGTTGCAAATTTAGATGATAAAGATGAACCAGATATGGCGAATAAATATAAAAA
GTCAATTATTATCGAACGAGCAGGTAGAAAGATTGGTTTAATTGGAGCACTTGTGGTTGC
AACTTTGGAAATTTCGAGTCCAGGAAATTTAAAAATTCTAGATGAAATTGAATCTGTCAA
ACAGGAAGCAGCAAGATTAAAAAGAGAAGATGACGTTGATATTATTATTGTTTTGTCACA
TTGTGGCCTTGTAATTGATCGTGAAATGGCACAAAGTGGTGATGAAATAGATATTATTGT
AGGTGGACATAGTCATTCTCTTCTTTATACAGGTAAATATTATCAATGAAAAACTTTTTC
AAGTGGATGTTCCTTTATGCGTGTATTCTTATCTTAGGTGATAATGTTCCCGGACCAGAT
GTTCCCGCAGATAAATATCCAATTGAATACAAACATGAAAGCGGACATAAAACAATTATC
GTACAAGCTTCAGCTTATACAAAATATCTCGGTGATTTAACAGTTTATTTTGATGAGAAC
GGAGAAATTGAGACATATGAAGGAAATACAATTTTCTTAGAAACAAGCATTGAACCTGAT
CCTGAAATTGTTGAACAATTAGCACCATGGAAAGCTGCAGTCGATGCAATTGGACAAAGA
AAAGTCGGTGATATCAAATCGATGCTGTATCAAAAAGATTGCGCTTTCGGAGAATGTAAT
ATTGGCGATTTTGTGTGTGATGCTTTTATTAATTACATCGTGACACATGAAGATTATCAA
GTTAATGATGGATGGGCATATGCAGCTATTGCAATTATTAATGCTGGTAGTATTCGCAAT
AATTTACCACCAGGTGAATTAATCTTTGATGATCTTTTCACTACAATTCCATTTATCAAC
ACACTCGATGTTTTTGAGTTGCTTGGTCAAGACTTGTTGGATGCACTTCAATTCTCTGGT
AATGCTTATAGATATTACAACTTTTTGCAATTTTCTGGATTAAAAGTAACATACAACATT
TCAATGCCAATGAATCAGCGAGTTATTTCAGTAGATGTTTTATGTCGAGAATGTGAAATT
CCAAAATATGAGAAATTGGATGTAACAAAATATTATCGTTTAATTATTTCAAGTTTTTTC
GGTGATGGTGGAAATGGATATTTGATGTTTAAAAACAAAAGAAATACCCGCACAACTAAA
GAAAAAGATATTGAAATTATAGAGCAATATTTCAAAAAGATGAGTCCCGTATTGCAGAAA
AAGGATGGAAGAATAAAAGTTTTAACTTGATCATGTAGTCAAATTATCCTATTATAATCA
AGAATAAAGATTTCTTACTTGTAAAAATATGCATAAACTTAAACTCTTGTATGTTTCTTA
TTGACGTGTATTTTTTTTATTATTTTAACATCATCTGCTACGTCCACACATATAATTTAG
GATAATTAGTCTTCTTTCACGAATTTGCCATTATAGAATGGCATAAATTGAAGAATGAGT
TGCCAATCTGTGATATAAAGCAAAGCCAAACTTCCAGCTGTTCCGAATGTTGCTAAACTA
CCAACCCTTT
>g2835.t2 Gene=g2835 Length=314
MKNFFKWMFLYACILILGDNVPGPDVPADKYPIEYKHESGHKTIIVQASAYTKYLGDLTV
YFDENGEIETYEGNTIFLETSIEPDPEIVEQLAPWKAAVDAIGQRKVGDIKSMLYQKDCA
FGECNIGDFVCDAFINYIVTHEDYQVNDGWAYAAIAIINAGSIRNNLPPGELIFDDLFTT
IPFINTLDVFELLGQDLLDALQFSGNAYRYYNFLQFSGLKVTYNISMPMNQRVISVDVLC
RECEIPKYEKLDVTKYYRLIISSFFGDGGNGYLMFKNKRNTRTTKEKDIEIIEQYFKKMS
PVLQKKDGRIKVLT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g2835.t2 | Gene3D | G3DSA:3.60.21.10 | - | 14 | 103 | 7.0E-17 |
| 11 | g2835.t2 | Gene3D | G3DSA:3.90.780.10 | - | 108 | 313 | 2.7E-51 |
| 2 | g2835.t2 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 17 | 312 | 4.1E-93 |
| 3 | g2835.t2 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 17 | 312 | 4.1E-93 |
| 6 | g2835.t2 | PRINTS | PR01607 | Apyrase family signature | 43 | 63 | 2.2E-12 |
| 4 | g2835.t2 | PRINTS | PR01607 | Apyrase family signature | 171 | 194 | 2.2E-12 |
| 5 | g2835.t2 | PRINTS | PR01607 | Apyrase family signature | 250 | 269 | 2.2E-12 |
| 1 | g2835.t2 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 107 | 276 | 2.2E-33 |
| 13 | g2835.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 28 | - |
| 14 | g2835.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 8 | - |
| 15 | g2835.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 9 | 17 | - |
| 16 | g2835.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 28 | - |
| 12 | g2835.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 29 | 314 | - |
| 8 | g2835.t2 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 24 | 100 | 1.71E-12 |
| 7 | g2835.t2 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 105 | 313 | 8.63E-48 |
| 9 | g2835.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
