General information for g2835.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Apyrase.
Parent gene
- The parent gene g2835 is located on chr_3: 20500421..20502281 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2835 | g2835.t8 | TTS | g2835.t8 | 20500351 | 20500351 |
| chr_3 | g2835 | g2835.t8 | isoform | g2835.t8 | 20500421 | 20502281 |
| chr_3 | g2835 | g2835.t8 | exon | g2835.t8.exon1 | 20500421 | 20501313 |
| chr_3 | g2835 | g2835.t8 | cds | g2835.t8.CDS1 | 20500421 | 20501313 |
| chr_3 | g2835 | g2835.t8 | exon | g2835.t8.exon2 | 20501379 | 20501774 |
| chr_3 | g2835 | g2835.t8 | cds | g2835.t8.CDS2 | 20501379 | 20501774 |
| chr_3 | g2835 | g2835.t8 | exon | g2835.t8.exon3 | 20501830 | 20502281 |
| chr_3 | g2835 | g2835.t8 | cds | g2835.t8.CDS3 | 20501830 | 20501995 |
| chr_3 | g2835 | g2835.t8 | TSS | g2835.t8 | 20502370 | 20502370 |
Sequences
>g2835.t8 Gene=g2835 Length=1741
ATGAAATTTATATTATGTTCGACAATTTTCATTCTTTATATTGGTTCTTGGATAATTTCA
AGTGAATCTGCTGCAGTTATTAAGAAAAATGAAAATCTTTTTCCACTTTCAATAATTCAT
GTTAATGATATTCATGCAAAGTAAGTGCACACACGTGTGACCTTTAAAATGTTAAATTGT
TATGATCATTACATACTTTTAAATTAGATTTGATGAAACTAATGTCGATAGTACAAGTTG
CAAGGAAAAATTAGGACAAGTATGCATAGGAGGATTTGCTCGTGTTATGACAATGGTTAA
GAAACTTAAAGCTGAAAGAGAGGCACAAAACAAAAATCCAATTTTTTTGAACATTGGTGA
TAATTTCGTTGGAACTTTATGGTATGAATTATTTGGATGGAATATTACATCAACTTTCTT
AAACATTCTTCCTGCAGACGCTACCACTTTAGGAAATCATGAATTCGATAGAGGTGTACC
TGAGGTTGTGCATTTTCTTGAACATTTAATTTCGCCTGTAGTTGTTGCAAATTTAGATGA
TAAAGATGAACCAGATATGGCGAATAAATATAAAAAGTCAATTATTATCGAACGAGCAGG
TAGAAAGATTGGTTTAATTGGAGCACTTGTGGTTGCAACTTTGGAAATTTCGAGTCCAGG
AAATTTAAAAATTCTAGATGAAATTGAATCTGTCAAACAGGAAGCAGCAAGATTAAAAAG
AGAAGATGACGTTGATATTATTATTGTTTTGTCACATTGTGGCCTTGTAATTGATCGTGA
AATGGCACAAAGTGGTGATGAAATAGATATTATTGTAGGTGGACATAGTCATTCTCTTCT
TTATACAGGTGATAATGTTCCCGGACCAGATGTTCCCGCAGATAAATATCCAATTGAATA
CAAACATGAAAGCGGACATAAAACAATTATCGTACAAGCTTCAGCTTATACAAAATATCT
CGGTGATTTAACAGTTTATTTTGATGAGAACGGAGAAATTGAGACATATGAAGGAAATAC
AATTTTCTTAGAAACAAGCATTGAACCTGATCCTGAAATTGTTGAACAATTAGCACCATG
GAAAGCTGCAGTCGATGCAATTGGACAAAGAAAAGTCGGTGATATCAAATCGATGCTGTA
TCAAAAAGATTGCGCTTTCGGAGAATGTAATATTGGCGATTTTGTGTGTGATGCTTTTAT
TAATTACATCGTGACACATGAAGATTATCAAGTTAATGATGGATGGGCATATGCAGCTAT
TGCAATTATTAATGCTGGTAGTATTCGCAATAATTTACCACCAGGTGAATTAATCTTTGA
TGATCTTTTCACTACAATTCCATTTATCAACACACTCGATGTTTTTGAGTTGCTTGGTCA
AGACTTGTTGGATGCACTTCAATTCTCTGGTAATGCTTATAGATATTACAACTTTTTGCA
ATTTTCTGGATTAAAAGTAACATACAACATTTCAATGCCAATGAATCAGCGAGTTATTTC
AGTAGATGTTTTATGTCGAGAATGTGAAATTCCAAAATATGAGAAATTGGATGTAACAAA
ATATTATCGTTTAATTATTTCAAGTTTTTTCGGTGATGGTGGAAATGGATATTTGATGTT
TAAAAACAAAAGAAATACCCGCACAACTAAAGAAAAAGATATTGAAATTATAGAGCAATA
TTTCAAAAAGATGAGTCCCGTATTGCAGAAAAAGGATGGAAGAATAAAAGTTTTAACTTG
A
>g2835.t8 Gene=g2835 Length=484
MTMVKKLKAEREAQNKNPIFLNIGDNFVGTLWYELFGWNITSTFLNILPADATTLGNHEF
DRGVPEVVHFLEHLISPVVVANLDDKDEPDMANKYKKSIIIERAGRKIGLIGALVVATLE
ISSPGNLKILDEIESVKQEAARLKREDDVDIIIVLSHCGLVIDREMAQSGDEIDIIVGGH
SHSLLYTGDNVPGPDVPADKYPIEYKHESGHKTIIVQASAYTKYLGDLTVYFDENGEIET
YEGNTIFLETSIEPDPEIVEQLAPWKAAVDAIGQRKVGDIKSMLYQKDCAFGECNIGDFV
CDAFINYIVTHEDYQVNDGWAYAAIAIINAGSIRNNLPPGELIFDDLFTTIPFINTLDVF
ELLGQDLLDALQFSGNAYRYYNFLQFSGLKVTYNISMPMNQRVISVDVLCRECEIPKYEK
LDVTKYYRLIISSFFGDGGNGYLMFKNKRNTRTTKEKDIEIIEQYFKKMSPVLQKKDGRI
KVLT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g2835.t8 | CDD | cd07409 | MPP_CD73_N | 1 | 248 | 9.0573E-117 |
| 14 | g2835.t8 | Coils | Coil | Coil | 126 | 146 | - |
| 12 | g2835.t8 | Gene3D | G3DSA:3.60.21.10 | - | 1 | 282 | 5.9E-77 |
| 13 | g2835.t8 | Gene3D | G3DSA:3.90.780.10 | - | 283 | 483 | 8.5E-51 |
| 3 | g2835.t8 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 2 | 482 | 2.2E-174 |
| 4 | g2835.t8 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 2 | 482 | 2.2E-174 |
| 6 | g2835.t8 | PRINTS | PR01607 | Apyrase family signature | 147 | 164 | 1.2E-25 |
| 8 | g2835.t8 | PRINTS | PR01607 | Apyrase family signature | 166 | 189 | 1.2E-25 |
| 5 | g2835.t8 | PRINTS | PR01607 | Apyrase family signature | 213 | 233 | 1.2E-25 |
| 7 | g2835.t8 | PRINTS | PR01607 | Apyrase family signature | 341 | 364 | 1.2E-25 |
| 9 | g2835.t8 | PRINTS | PR01607 | Apyrase family signature | 420 | 439 | 1.2E-25 |
| 2 | g2835.t8 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 14 | 183 | 4.7E-7 |
| 1 | g2835.t8 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 277 | 446 | 6.0E-33 |
| 11 | g2835.t8 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 2 | 272 | 2.05E-70 |
| 10 | g2835.t8 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 275 | 483 | 2.75E-47 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed