General information for g5157.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Myosin heavy chain, muscle.
Parent gene
- The parent gene g5157 is located on chr_2: 7349389..7353465 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5157 | g5157.t16 | isoform | g5157.t16 | 7343414 | 7351406 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon1 | 7343414 | 7343584 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS1 | 7343414 | 7343584 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon2 | 7344169 | 7344576 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS2 | 7344169 | 7344576 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon3 | 7346722 | 7346825 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS3 | 7346722 | 7346825 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon4 | 7348795 | 7348957 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS4 | 7348795 | 7348957 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon5 | 7349015 | 7349395 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS5 | 7349015 | 7349395 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon6 | 7350463 | 7350606 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS6 | 7350463 | 7350606 |
| chr_2 | g5157 | g5157.t16 | exon | g5157.t16.exon7 | 7351200 | 7351406 |
| chr_2 | g5157 | g5157.t16 | cds | g5157.t16.CDS7 | 7351200 | 7351406 |
| chr_2 | g5157 | g5157.t16 | TSS | g5157.t16 | NA | NA |
| chr_2 | g5157 | g5157.t16 | TTS | g5157.t16 | NA | NA |
Sequences
>g5157.t16 Gene=g5157 Length=1578
ATGCCAAAGGTTATCAAGCAAGAAGGTGAAGACCCAGATCCCTCACCATGGTTGTTCGTC
TCACTCGAACAAAAGCGTATCGATCAATCGAAACCATACGATGCCAAGAAAGCCTGCTGG
GTTCCAGATGAAAAGGAGGGCTACGTCTTGGGTGAAATTAAGTCAACCAAGGGAGACTTA
GTCACCGTTTCTGTTCCCGGTGGCGAGACTAAGGATTTAAAGAAAGACTTGGTTGCACAA
GTCAACCCTCCAAAGTACGAAAAATGCGAGGACATGTCTAACTTGACATACCTCAACGAT
GCCTCGGTTCTCCATAACTTGAGACAACGTTATCTTGCCAAACTTATCTACACCTACTCT
GGCCTCTTCTGTATTGTAATCAACCCTTACAAACGTTGGCCATTGTATACCAACCGCGTC
GCTAAAATGTACCGTGGAAAGCGTCGCGCTGAAATGCCACCTCATCTTTTCTCAATTTCT
GACGGTGCCTACGTCAACATGTTGACAGATCATGAAAATCAATCCATGTTGATTACTGGT
GAGTCAGGAGCAGGAAAAACTGAGAACACGAAGAAGGTTATTGCCTACTTCGCCACTATT
GGTGCATCAACCAAGAAATCCGAAAGTGACGAGAAGAAGGGATCACTTGAAGATCAAGTC
GTCCAAACCAATCCCGTACTTGAAGCTTTCGGTAACGCCAAGACCGTCCGTAATGATAAC
TCATCACGTTTCGGTAAATTCATCCGTATTCACTTCTCTGGATCGGGTAAATTGTCAGGT
GCTGATATTGAAACCTACTTGTTGGAGAAGGCTCGTGTCATCTCACAACAATCATTGGAA
CGTTCATATCACATCTTCTACCAAATGATGTCAGGCTCAGTTCCAGGTCTTAAAGACATG
TGTATTTTATCCAACAACATTATGGATTATCATATTGTATCCCAGGGCAAAACCACCATC
CCAAATGTTGATGATGGTGAAGAATGTGAACTTACTGATCAAGCCTTCGATGTCTTGGGT
TTCACACAAGAAGAGAAGGACAACATCTACAAGATTGTCGCCGCTGTCATGCATATGGGT
GCTATGCAATTCAAACAAAGAGGTCGCGAAGAACAGGCTGAAGCTGATGGTACTGAAGCT
GGTGAGAAAGTCGCCAAATTGCTCGGTGTTGACACACAAGATCTCTACAAGAACTTGCTT
AAGCCACGTATTAAGGTCGGTAACGAGTTCGTCACACAAGGTCGTAACAAAGATCAAGTA
TACAACTCAGTTGGTGCCCTTTGCAAGGGTGTCTTCGATCGTCTCTTCAAATGGCTCGTC
AAGAAGTGTAACGAAACACTTGACACCAAGCAAAAACGTGTTCACTTCATTGGTGTGTTG
GATATTGCTGGTTTCGAAATTTTCGACTACAACGGTTTTGAACAATTGTGTATTAACTTC
ACCAATGAAAAATTGCAACAATTCTTTAACCATCACATGTTCGTTCTTGAACAAGAGGAA
TATAAGAAAGAAGGTATCGACTGGGCTTTCATTGATTTCGGCATGGACTTGTTGGCTTGT
ATTGAGATGATTGAGAAG
>g5157.t16 Gene=g5157 Length=526
MPKVIKQEGEDPDPSPWLFVSLEQKRIDQSKPYDAKKACWVPDEKEGYVLGEIKSTKGDL
VTVSVPGGETKDLKKDLVAQVNPPKYEKCEDMSNLTYLNDASVLHNLRQRYLAKLIYTYS
GLFCIVINPYKRWPLYTNRVAKMYRGKRRAEMPPHLFSISDGAYVNMLTDHENQSMLITG
ESGAGKTENTKKVIAYFATIGASTKKSESDEKKGSLEDQVVQTNPVLEAFGNAKTVRNDN
SSRFGKFIRIHFSGSGKLSGADIETYLLEKARVISQQSLERSYHIFYQMMSGSVPGLKDM
CILSNNIMDYHIVSQGKTTIPNVDDGEECELTDQAFDVLGFTQEEKDNIYKIVAAVMHMG
AMQFKQRGREEQAEADGTEAGEKVAKLLGVDTQDLYKNLLKPRIKVGNEFVTQGRNKDQV
YNSVGALCKGVFDRLFKWLVKKCNETLDTKQKRVHFIGVLDIAGFEIFDYNGFEQLCINF
TNEKLQQFFNHHMFVLEQEEYKKEGIDWAFIDFGMDLLACIEMIEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g5157.t16 | Coils | Coil | Coil | 521 | 526 | - |
| 13 | g5157.t16 | Gene3D | G3DSA:2.30.30.360 | Myosin S1 fragment | 32 | 82 | 3.4E-22 |
| 12 | g5157.t16 | Gene3D | G3DSA:3.40.850.10 | Kinesin | 83 | 319 | 2.5E-95 |
| 11 | g5157.t16 | Gene3D | G3DSA:1.20.120.720 | - | 342 | 466 | 3.4E-44 |
| 14 | g5157.t16 | Gene3D | G3DSA:1.20.58.530 | - | 467 | 526 | 2.2E-28 |
| 3 | g5157.t16 | PANTHER | PTHR45615:SF27 | MYOSIN HEAVY CHAIN, MUSCLE | 1 | 526 | 0.0 |
| 4 | g5157.t16 | PANTHER | PTHR45615 | MYOSIN HEAVY CHAIN, NON-MUSCLE | 1 | 526 | 0.0 |
| 5 | g5157.t16 | PRINTS | PR00193 | Myosin heavy chain signature | 117 | 136 | 3.5E-63 |
| 9 | g5157.t16 | PRINTS | PR00193 | Myosin heavy chain signature | 173 | 198 | 3.5E-63 |
| 8 | g5157.t16 | PRINTS | PR00193 | Myosin heavy chain signature | 225 | 252 | 3.5E-63 |
| 7 | g5157.t16 | PRINTS | PR00193 | Myosin heavy chain signature | 456 | 484 | 3.5E-63 |
| 6 | g5157.t16 | PRINTS | PR00193 | Myosin heavy chain signature | 510 | 526 | 3.5E-63 |
| 2 | g5157.t16 | Pfam | PF02736 | Myosin N-terminal SH3-like domain | 36 | 75 | 3.7E-14 |
| 1 | g5157.t16 | Pfam | PF00063 | Myosin head (motor domain) | 90 | 526 | 1.4E-193 |
| 18 | g5157.t16 | ProSiteProfiles | PS51844 | Myosin N-terminal SH3-like domain profile. | 34 | 83 | 18.222 |
| 17 | g5157.t16 | ProSiteProfiles | PS51456 | Myosin motor domain profile. | 87 | 526 | 171.622 |
| 16 | g5157.t16 | SMART | SM00242 | MYSc_2a | 81 | 526 | 5.1E-158 |
| 10 | g5157.t16 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 58 | 526 | 8.06E-186 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5157/g5157.t16; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5157.t16.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0051015 | actin filament binding | MF |
| GO:0005524 | ATP binding | MF |
| GO:0016459 | myosin complex | CC |
| GO:0003774 | cytoskeletal motor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed