Gene loci information

Transcript annotation

  • This transcript has been annotated as Myosin heavy chain, muscle.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5157 g5157.t7 isoform g5157.t7 7338954 7351406
chr_2 g5157 g5157.t7 exon g5157.t7.exon1 7338954 7339077
chr_2 g5157 g5157.t7 cds g5157.t7.CDS1 7338954 7339077
chr_2 g5157 g5157.t7 exon g5157.t7.exon2 7340085 7340677
chr_2 g5157 g5157.t7 cds g5157.t7.CDS2 7340085 7340677
chr_2 g5157 g5157.t7 exon g5157.t7.exon3 7343414 7343584
chr_2 g5157 g5157.t7 cds g5157.t7.CDS3 7343414 7343584
chr_2 g5157 g5157.t7 exon g5157.t7.exon4 7344169 7344576
chr_2 g5157 g5157.t7 cds g5157.t7.CDS4 7344169 7344576
chr_2 g5157 g5157.t7 exon g5157.t7.exon5 7345295 7345348
chr_2 g5157 g5157.t7 cds g5157.t7.CDS5 7345295 7345348
chr_2 g5157 g5157.t7 exon g5157.t7.exon6 7345666 7345821
chr_2 g5157 g5157.t7 cds g5157.t7.CDS6 7345666 7345821
chr_2 g5157 g5157.t7 exon g5157.t7.exon7 7345998 7346051
chr_2 g5157 g5157.t7 cds g5157.t7.CDS7 7345998 7346051
chr_2 g5157 g5157.t7 exon g5157.t7.exon8 7346722 7346825
chr_2 g5157 g5157.t7 cds g5157.t7.CDS8 7346722 7346825
chr_2 g5157 g5157.t7 exon g5157.t7.exon9 7348795 7348957
chr_2 g5157 g5157.t7 cds g5157.t7.CDS9 7348795 7348957
chr_2 g5157 g5157.t7 exon g5157.t7.exon10 7349015 7349395
chr_2 g5157 g5157.t7 cds g5157.t7.CDS10 7349015 7349395
chr_2 g5157 g5157.t7 exon g5157.t7.exon11 7350463 7350606
chr_2 g5157 g5157.t7 cds g5157.t7.CDS11 7350463 7350606
chr_2 g5157 g5157.t7 exon g5157.t7.exon12 7350925 7351068
chr_2 g5157 g5157.t7 cds g5157.t7.CDS12 7350925 7351068
chr_2 g5157 g5157.t7 exon g5157.t7.exon13 7351200 7351406
chr_2 g5157 g5157.t7 cds g5157.t7.CDS13 7351200 7351406
chr_2 g5157 g5157.t7 TSS g5157.t7 NA NA
chr_2 g5157 g5157.t7 TTS g5157.t7 NA NA

Sequences

>g5157.t7 Gene=g5157 Length=2703
ATGCCAAAGGTTATCAAGCAAGAAGGTGAAGACCCAGATCCCTCACCATGGTTGTTCGTC
TCACTCGAACAAAAGCGTATCGATCAATCGAAACCATACGATGCCAAGAAAGCCTGCTGG
GTTCCAGATGAAAAGGAGGGCTACGTCTTGGGTGAAATTAAGTCAACCAAGGGAGACTTA
GTCACCGTTTCTGTTCCCGGTGGCGAGGAACGAAATCTGAAACGTGATTTGATTCATCCA
GTCAATCCACCAAAATTCGAAAAAGTCGAGGATATGGCCGATTTGACCCATCTTAATGAA
GGTGCCGTGTTGCATAATCTGCGTCAGCGGTACTATTCTCGACTCATCTATACTAAGGAT
TTAAAGAAAGACTTGGTTGCACAAGTCAACCCTCCAAAGTACGAAAAATGCGAGGACATG
TCTAACTTGACATACCTCAACGATGCCTCGGTTCTCCATAACTTGAGACAACGTTATCTT
GCCAAACTTATCTACACCTACTCTGGCCTCTTCTGTATTGTAATCAACCCTTACAAACGT
TGGCCATTGTATACCAACCGCGTCGCTAAAATGTACCGTGGAAAGCGTCGCGCTGAAATG
CCACCTCATCTTTTCTCAATTTCTGACGGTGCCTACGTCAACATGTTGACAGATCATGAA
AATCAATCCATGTTGATTACTGGTGAGTCAGGAGCAGGAAAAACTGAGAACACGAAGAAG
GTTATTGCCTACTTCGCCACTATTGGTGCATCAACCAAGAAATCCGAAAGTGACGAGAAG
AAGGGATCACTTGAAGATCAAGTCGTCCAAACCAATCCCGTACTTGAAGCTTTCGGTAAC
GCCAAGACCGTCCGTAATGATAACTCATCACGTTTCGGTAAATTCATCCGTATTCACTTC
TCTGGATCGGGTAAATTGTCAGGTGCTGATATTGAAACCTACTTGTTGGAGAAGGCTCGT
GTCATCTCACAACAATCATTGGAACGTTCATATCACATCTTCTACCAAATGATGTCAGGC
TCAGTTCCAGGTCTTAAAGACATGTGTATTTTATCCAACAACATTATGGATTATCATATT
GTATCCCAGGGCAAAACCACCATCCCAAATGTTGATGATGGTGAAGAATGTGAACTTACT
GATGGCAAGACAACAATTCCAAGTGTTGATGATAGTGAAGAAATGCAATTAACCGATAAT
CGAGCTGTTAAAATAGTAAATAATAATGACAATGATAAATTAAATTTAGAAAAAACATTA
TTATCATCGCGAATGAGCGATTATCCGACAATAACACAAGGCAAAACACGTATTCCTGGC
ATTAATGATGGTGAAGAGTTTGAAATTACTGATGGAAAAATAACAATCCCTGGCATCGAT
GACGGTGAAGAATGTCAACTTGCTGATCAAGCCTTCGATGTCTTGGGTTTCACACAAGAA
GAGAAGGACAACATCTACAAGATTGTCGCCGCTGTCATGCATATGGGTGCTATGCAATTC
AAACAAAGAGGTCGCGAAGAACAGGCTGAAGCTGATGGTACTGAAGCTGGTGAGAAAGTC
GCCAAATTGCTCGGTGTTGACACACAAGATCTCTACAAGAACTTGCTTAAGCCACGTATT
AAGGTCGGTAACGAGTTCGTCACACAAGGTCGTAACAAAGATCAAGTATACAACTCAGTT
GGTGCCCTTTGCAAGGGTGTCTTCGATCGTCTCTTCAAATGGCTCGTCAAGAAGTGTAAC
GAAACACTTGACACCAAGCAAAAACGTGTTCACTTCATTGGTGTGTTGGATATTGCTGGT
TTCGAAATTTTCGACTACAACGGTTTTGAACAATTGTGTATTAACTTCACCAATGAAAAA
TTGCAACAATTCTTTAACCATCACATGTTCGTTCTTGAACAAGAGGAATATAAGAAAGAA
GGTATCGACTGGGCTTTCATTGATTTCGGCATGGACTTGTTGGCTTGTATTGAGATGATT
GAGAAGCCTATGGGTATCTTGTCAATTCTTGAAGAAGAATCTATGTTCCCAAAAGCTACT
GATCAAACATTCGCTGAGAAATTGATGGTCAACCATTTGGGTAAATCAGCTCCATTCTTG
AAGCCAAAACCACCAAAGCCAGGTTGCCAAGCTGGTCACTTTGCTATTGCTCACTACGCT
GGTGTTGTCACATATAACATCACCGGTTGGTTGGAAAAGAACAAGGATCCATTGAATGAC
ACAGTCGTCGATCAATTCAAGAAGGGTGCCAATAATCTTTTGGTTGAAATCTTTGCTGAT
CATCCAGGTCAATCAGCACCAGCTGATGCTGGCGGCAAGGGTGGTCGTGGTAAGAAAGGT
GGTGGTTTCGCCACTGTCTCATCATCATACCGTGAACAGTTGAACAACTTGATGACAACA
TTGCGTTCAACACATCCTCATTTCGTCCGTTGCATTATTCCAAATGAATTGAAACAGACT
GGTCTCATCGATGCCAAGCTTGTTATGCATCAGCTCACTTGTAACGGTGTACTTGAAGGT
ATCCGTATCTGTCGTAAAGGTTTCCCCAACAGAATGAACTATCCAGATTTCAAATTGCGT
TACCAAATTCTTGCACCTGCACTCATGCAAGCTGAAAAAGACCCTAAGAAGGCAGCAGCC
GCATGTTTGGACGCAATTAAATTGGATCCAGAAAGTTTCCGATTGGGTCATACTAAGGTC
TAG

>g5157.t7 Gene=g5157 Length=900
MPKVIKQEGEDPDPSPWLFVSLEQKRIDQSKPYDAKKACWVPDEKEGYVLGEIKSTKGDL
VTVSVPGGEERNLKRDLIHPVNPPKFEKVEDMADLTHLNEGAVLHNLRQRYYSRLIYTKD
LKKDLVAQVNPPKYEKCEDMSNLTYLNDASVLHNLRQRYLAKLIYTYSGLFCIVINPYKR
WPLYTNRVAKMYRGKRRAEMPPHLFSISDGAYVNMLTDHENQSMLITGESGAGKTENTKK
VIAYFATIGASTKKSESDEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHF
SGSGKLSGADIETYLLEKARVISQQSLERSYHIFYQMMSGSVPGLKDMCILSNNIMDYHI
VSQGKTTIPNVDDGEECELTDGKTTIPSVDDSEEMQLTDNRAVKIVNNNDNDKLNLEKTL
LSSRMSDYPTITQGKTRIPGINDGEEFEITDGKITIPGIDDGEECQLADQAFDVLGFTQE
EKDNIYKIVAAVMHMGAMQFKQRGREEQAEADGTEAGEKVAKLLGVDTQDLYKNLLKPRI
KVGNEFVTQGRNKDQVYNSVGALCKGVFDRLFKWLVKKCNETLDTKQKRVHFIGVLDIAG
FEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWAFIDFGMDLLACIEMI
EKPMGILSILEEESMFPKATDQTFAEKLMVNHLGKSAPFLKPKPPKPGCQAGHFAIAHYA
GVVTYNITGWLEKNKDPLNDTVVDQFKKGANNLLVEIFADHPGQSAPADAGGKGGRGKKG
GGFATVSSSYREQLNNLMTTLRSTHPHFVRCIIPNELKQTGLIDAKLVMHQLTCNGVLEG
IRICRKGFPNRMNYPDFKLRYQILAPALMQAEKDPKKAAAACLDAIKLDPESFRLGHTKV

Protein features from InterProScan

Transcript Database ID Name Start End E.value
25 g5157.t7 CDD cd14909 MYSc_Myh1_insects_crustaceans 149 900 0.0000000
23 g5157.t7 Gene3D G3DSA:2.30.30.360 Myosin S1 fragment 32 82 0.0000000
19 g5157.t7 Gene3D G3DSA:3.40.850.10 Kinesin 83 123 0.0000000
20 g5157.t7 Gene3D G3DSA:3.40.850.10 Kinesin 124 423 0.0000000
18 g5157.t7 Gene3D G3DSA:1.10.10.820 - 449 476 0.0000082
17 g5157.t7 Gene3D G3DSA:1.20.120.720 - 478 602 0.0000000
24 g5157.t7 Gene3D G3DSA:1.20.58.530 - 603 759 0.0000000
21 g5157.t7 Gene3D G3DSA:3.40.850.10 Kinesin 782 847 0.0000000
22 g5157.t7 Gene3D G3DSA:1.20.58.1940 - 848 900 0.0000000
5 g5157.t7 PANTHER PTHR45615:SF27 MYOSIN HEAVY CHAIN, MUSCLE 1 118 0.0000000
8 g5157.t7 PANTHER PTHR45615 MYOSIN HEAVY CHAIN, NON-MUSCLE 1 118 0.0000000
6 g5157.t7 PANTHER PTHR45615:SF27 MYOSIN HEAVY CHAIN, MUSCLE 119 381 0.0000000
9 g5157.t7 PANTHER PTHR45615 MYOSIN HEAVY CHAIN, NON-MUSCLE 119 381 0.0000000
4 g5157.t7 PANTHER PTHR45615:SF27 MYOSIN HEAVY CHAIN, MUSCLE 450 900 0.0000000
7 g5157.t7 PANTHER PTHR45615 MYOSIN HEAVY CHAIN, NON-MUSCLE 450 900 0.0000000
10 g5157.t7 PRINTS PR00193 Myosin heavy chain signature 165 184 0.0000000
11 g5157.t7 PRINTS PR00193 Myosin heavy chain signature 221 246 0.0000000
14 g5157.t7 PRINTS PR00193 Myosin heavy chain signature 273 300 0.0000000
13 g5157.t7 PRINTS PR00193 Myosin heavy chain signature 592 620 0.0000000
12 g5157.t7 PRINTS PR00193 Myosin heavy chain signature 646 674 0.0000000
3 g5157.t7 Pfam PF02736 Myosin N-terminal SH3-like domain 36 74 0.0000000
2 g5157.t7 Pfam PF00063 Myosin head (motor domain) 138 397 0.0000000
1 g5157.t7 Pfam PF00063 Myosin head (motor domain) 450 900 0.0000000
28 g5157.t7 ProSiteProfiles PS51844 Myosin N-terminal SH3-like domain profile. 34 83 18.5710000
27 g5157.t7 ProSiteProfiles PS51456 Myosin motor domain profile. 135 900 241.2780000
26 g5157.t7 SMART SM00242 MYSc_2a 129 900 0.0000000
15 g5157.t7 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 39 118 0.0000000
16 g5157.t7 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 130 900 0.0000000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5157/g5157.t7; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5157.t7.fa.iupred3.txt does not exist

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0051015 actin filament binding MF
GO:0005524 ATP binding MF
GO:0016459 myosin complex CC
GO:0003774 cytoskeletal motor activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed