General information for g9330.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 12a5, mitochondrial.
Parent gene
- The parent gene g9330 is located on chr_1: 1876646..1879020 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9330 | g9330.t1 | isoform | g9330.t1 | 1876646 | 1879020 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon1 | 1876646 | 1876921 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS1 | 1876646 | 1876921 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon2 | 1876984 | 1877213 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS2 | 1876984 | 1877213 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon3 | 1877307 | 1877582 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS3 | 1877307 | 1877582 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon4 | 1878078 | 1878234 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS4 | 1878078 | 1878234 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon5 | 1878358 | 1878773 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS5 | 1878358 | 1878773 |
| chr_1 | g9330 | g9330.t1 | exon | g9330.t1.exon6 | 1878858 | 1879020 |
| chr_1 | g9330 | g9330.t1 | cds | g9330.t1.CDS6 | 1878858 | 1879020 |
| chr_1 | g9330 | g9330.t1 | TTS | g9330.t1 | 1879051 | 1879051 |
| chr_1 | g9330 | g9330.t1 | TSS | g9330.t1 | NA | NA |
Sequences
>g9330.t1 Gene=g9330 Length=1518
ATGTTGAAGAAAATTTTCATTAATTCTTCAATTTTATTTTCTGTTCGTAATTCGTCAACA
ATTGCACAGAAAAATCCAAAACCATTTTCGAAAATTCCAACTGTATCGATTTTCAAGCTT
ATTACCGGAAGTTTACCTGGAGGAAAATATTATAAAAAATCGTTATTAGATATTGGAAAA
TCATTAAATGAAGAATATGGAGATATTTTAAGAATTCCATCAATTTTAGGCAATGATGAA
ATGATAATAATTTTTAAAGCTGATGACTTTGAAACAGTCTTCAGAAATGAAGGAATTTGG
CCAACTCGTAAAGCACTCGTAACATTGAAATATTATAGAGAAAAATATAAATCAGATATT
TATTCAAATTATGGAAGCATTTTAACAGGAGATGGTGAAAAATGGTACAAAACTAGAACA
ACTGTTAATCCAATTTTGCTCAAACCAAAAGTTGTAGAATCTTATAGAGGAATAATTGAT
GAAGTTGCTAAAGACCTTGTTGAAGAAATGAAAAACATAAGAAATGATAAAAGTGAAATG
CCTGGAAATTTTCTAGATATTATAGTTGCATGGTCAGCAGAATCAATTGTATCAGTTGTT
TTGGGAAAACGTCTCAACATGCTTTCTGGAAGATCAAATGATGAAAATGCACAGAAACTT
ATGAATTGTATAAGGCAATTTCTTGAACAAAGTGCTCAATTTGAGTTGGGAGTTTCACTA
TGGAGATATTATGAGTCAAAGGCATTCAAAGAATTAATAAAAGTTTTTGAGAATTTGACA
AATGTAACAAGTTATTTTGTTAATGAAGCATTAGAAAAATTTGAGAATGAAAAAGGTGAT
GAAAATGTTGAAAATTCTGTTTTTGTAAGTTTACTGAAAATAAACAAAGAAATAACAACA
GTTATGGTCATGGATGCTTTGACTGCTGGTGTTGATACTACTGCACTGATTTTAACAAAT
CTTCTTTACATGCTTGCTAAAAATCCTACAAAACAAGAAATACTTCGTAAAGAAATTTTC
CAATTTCTTCCTGAAAAATCTTCAATTCCAAGTTATGAAAACATGAAACAGTTGTCATAT
TTAAGAGCTTGTGTAAAAGAAACTTTTAGAACAATGCCATTAATTCTTGGAAGTAATCGA
GTTATTCAAAATGATGTAGTGCTTTCAGGCTATCAAGTTCCAAAAAATAAAAATATTGCA
TTGACATTTACACAGGAACTTGTCAATCCAAAATATTATCCTGAACCACAAAAATTTATT
CCAGAAAGATGGCTAAGAGACAGAACTGACAATAAATGTCCACATGCAACTGAAAGTAAT
CCTTTCTCTTTTATGCCTTTTGGTCATGGTAGCAGAAGTTGCATAGGAAAAAGAATTGCA
AACATGGAAATTGAGCTTGCAACTGCAAATATAATAAGAAATTTCAAATTAGAATGGCAC
TATCCTGATATTAAAATTAGAAATGCTTTGTTTAATATTCCTGATTCTGAAATGAAAATT
AAAGTTCTTGATGTTTAA
>g9330.t1 Gene=g9330 Length=505
MLKKIFINSSILFSVRNSSTIAQKNPKPFSKIPTVSIFKLITGSLPGGKYYKKSLLDIGK
SLNEEYGDILRIPSILGNDEMIIIFKADDFETVFRNEGIWPTRKALVTLKYYREKYKSDI
YSNYGSILTGDGEKWYKTRTTVNPILLKPKVVESYRGIIDEVAKDLVEEMKNIRNDKSEM
PGNFLDIIVAWSAESIVSVVLGKRLNMLSGRSNDENAQKLMNCIRQFLEQSAQFELGVSL
WRYYESKAFKELIKVFENLTNVTSYFVNEALEKFENEKGDENVENSVFVSLLKINKEITT
VMVMDALTAGVDTTALILTNLLYMLAKNPTKQEILRKEIFQFLPEKSSIPSYENMKQLSY
LRACVKETFRTMPLILGSNRVIQNDVVLSGYQVPKNKNIALTFTQELVNPKYYPEPQKFI
PERWLRDRTDNKCPHATESNPFSFMPFGHGSRSCIGKRIANMEIELATANIIRNFKLEWH
YPDIKIRNALFNIPDSEMKIKVLDV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g9330.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 21 | 504 | 2.3E-106 |
| 2 | g9330.t1 | PANTHER | PTHR24291:SF155 | CYTOCHROME P450 301B1 | 25 | 492 | 2.7E-109 |
| 3 | g9330.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 25 | 492 | 2.7E-109 |
| 8 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 1 | 18 | 1.8E-18 |
| 6 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 298 | 315 | 1.8E-18 |
| 10 | g9330.t1 | PRINTS | PR00385 | P450 superfamily signature | 309 | 326 | 4.8E-11 |
| 5 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 318 | 344 | 1.8E-18 |
| 13 | g9330.t1 | PRINTS | PR00385 | P450 superfamily signature | 363 | 374 | 4.8E-11 |
| 9 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 402 | 426 | 1.8E-18 |
| 7 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 444 | 454 | 1.8E-18 |
| 12 | g9330.t1 | PRINTS | PR00385 | P450 superfamily signature | 445 | 454 | 4.8E-11 |
| 4 | g9330.t1 | PRINTS | PR00463 | E-class P450 group I signature | 454 | 477 | 1.8E-18 |
| 11 | g9330.t1 | PRINTS | PR00385 | P450 superfamily signature | 454 | 465 | 4.8E-11 |
| 1 | g9330.t1 | Pfam | PF00067 | Cytochrome P450 | 45 | 480 | 7.4E-76 |
| 18 | g9330.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 19 | g9330.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 20 | g9330.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 15 | - |
| 21 | g9330.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 22 | - |
| 17 | g9330.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 505 | - |
| 22 | g9330.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 447 | 456 | - |
| 14 | g9330.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 41 | 502 | 2.88E-81 |
| 15 | g9330.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K17951 (all-trans-retinol 3,4-desaturase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed